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Plasmid Files

pCMV6-AN-YFP

PrecisionShuttle™ mammalian expression vector for fusing an ORF to an N-terminal TurboYFP tag.

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pCMV6-AN-YFP.dna
Map and Sequence File:    Download    Open   
Sequence Author:  OriGene
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DraIII (6434) ScaI (5762) AlwNI (4808) PciI (4392) BstZ17I (4013) BsmI (3961) AanI (3900) PfoI (3816) BstBI (3723) PflFI - Tth111I (3159) AanI (6559) NdeI (592) SnaBI (698) VP1.5 (forward primer) (839 .. 856) Eco53kI (924) SacI (926) EcoRI (979) BamHI (992) Acc65I (998) KpnI (1002) BspEI (1734) AsiSI - SgfI (1753) MreI (1755) AscI (1759) HindIII (1773) RsrII (1790) MluI (1796) NotI (1806) PaeR7I - PspXI - XhoI (1814) PmeI (1823) FseI (1833) XL39 (reverse primer) (1929 .. 1948) BbsI (2046) EcoO109I (2053) BbvCI - Bpu10I (2149) BsmBI (2213) BstXI (2300) AgeI (2411) XcmI (2506) SexAI * (2598) SfiI (2784) StuI (2830) AvrII (2831) BclI * (2882) BsaBI * (2900) PstI (3094) pCMV6-AN-YFP 6631 bp
DraIII  (6434)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
ScaI  (5762)
1 site
A G T A C T T C A T G A
AlwNI  (4808)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PciI  (4392)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BstZ17I  (4013)
1 site
G T A T A C C A T A T G
BsmI  (3961)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
AanI  (3900)
2 sites
T T A T A A A A T A T T
PfoI  (3816)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
BstBI  (3723)
1 site
T T C G A A A A G C T T
PflFI  (3159)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3159)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
AanI  (6559)
2 sites
T T A T A A A A T A T T
NdeI  (592)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (698)
1 site
T A C G T A A T G C A T
Eco53kI  (924)
1 site
G A G C T C C T C G A G
SacI  (926)
1 site
G A G C T C C T C G A G
EcoRI  (979)
1 site
G A A T T C C T T A A G
BamHI  (992)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
Acc65I  (998)
1 site
G G T A C C C C A T G G
KpnI