Resources
Plasmid Files

pCS6-Empty

Empty cloning vector for transient mammalian cell expression of a cDNA. Related to pCMV SPORT6.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pCS6-Empty Sequence and MappCS6-Empty.dna
Map and Sequence File   
Sequence Author:  transOMIC
Download Free Trial Get SnapGene Viewer

 AanI (3958) AanI (239) AanI (690) MluI (711) HindIII (717) PspOMI (723) ApaI (727) AbsI - PaeR7I - PspXI - XhoI (729) XbaI (741) EcoRV (749) TspMI - XmaI (752) SmaI (754) EcoRI (757) RsrII (764) AgeI (766) Acc65I (769) KpnI (773) SP6 promoter pCS6-Empty 4318 bp
AanI  (3958)
3 sites
T T A T A A A A T A T T
AanI  (239)
3 sites
T T A T A A A A T A T T
AanI  (690)
3 sites
T T A T A A A A T A T T
MluI  (711)
1 site
A C G C G T T G C G C A
HindIII  (717)
1 site
A A G C T T T T C G A A
PspOMI  (723)
1 site
G G G C C C C C C G G G
ApaI  (727)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
AbsI  (729)
1 site
C C T C G A G G G G A G C T C C
PaeR7I  (729)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (729)
1 site
V C T C G A G B B G A G C T C V
XhoI  (729)
1 site
C T C G A G G A G C T C
XbaI  (741)
1 site
T C T A G A A G A T C T
EcoRV  (749)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to
delete a base after cleavage.
TspMI  (752)
1 site
C C C G G G G G G C C C
XmaI  (752)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
SmaI  (754)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
EcoRI  (757)
1 site
G A A T T C C T T A A G
RsrII  (764)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII
recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
AgeI  (766)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
Acc65I  (769)
1 site
G G T A C C C C A T G G
KpnI  (773)
1 site
G G T A C C C C A T G G
AmpR
2714 .. 3574  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   2714 .. 3505  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
2714 .. 3574  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   3506 .. 3574  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
2714 .. 3574  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
ori
1913 .. 2501  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
1913 .. 2501  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
f1 ori
3862 .. 4317  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
3862 .. 4317  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
CMV enhancer
1150 .. 1453  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
1150 .. 1453  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
946 .. 1149  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
946 .. 1149  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
SV40 poly(A) signal
125 .. 259  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
125 .. 259  =  135 bp
SV40 polyadenylation signal
MCS
711 .. 774  =  64 bp
multiple cloning site
MCS
711 .. 774  =  64 bp
multiple cloning site
loxP
2579 .. 2612  =  34 bp
Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT).
loxP
2579 .. 2612  =  34 bp
Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT).
SP6 promoter
775 .. 793  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
SP6 promoter
775 .. 793  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
M13 fwd
664 .. 680  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 fwd
664 .. 680  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 rev
804 .. 820  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 rev
804 .. 820  =  17 bp
common sequencing primer, one of multiple similar
variants
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter