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pTRE3G

Vector for doxycycline-inducible expression of a gene in cells containing the Tet-On® 3G transactivator protein.

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pTRE3G.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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EcoRI (3426) AatII (3355) ZraI (3353) XmnI (3032) ScaI (2913) TatI (2911) FspI (2655) NmeAIII (2581) BglI (2553) BsrFI (2513) BsaI (2494) BmrI (2473) AhdI (2433) AlwNI (1956) PspFI (1848) BseYI (1844) AvaI - BsoBI - PaeR7I - PspXI - XhoI (1) BmeT110I (2) HindIII (253) KasI (274) NarI (275) SfoI (276) PluTI (278) Eco53kI (292) SacI (294) SalI (383) AccI (384) PspOMI (393) ApaI (397) BglII (399) BspDI * - ClaI * (406) EagI (411) EcoRV (419) MluI (423) NdeI (430) NheI (435) BmtI (439) PstI - SbfI (445) BamHI (447) BtgZI (484) PflMI (658) BseRI (764) StyI (806) BsgI (890) BsaBI * (1121) MfeI (1209) HpaI (1222) BspQI - SapI (1424) PciI (1540) NspI (1544) DrdI (1648) pTRE3G 3431 bp
EcoRI  (3426)
1 site
G A A T T C C T T A A G
AatII  (3355)
1 site
G A C G T C C T G C A G
ZraI  (3353)
1 site
G A C G T C C T G C A G
XmnI  (3032)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (2913)
1 site
A G T A C T T C A T G A
TatI  (2911)
1 site
W G T A C W W C A T G W
FspI  (2655)
1 site
T G C G C A A C G C G T
NmeAIII  (2581)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BglI  (2553)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BsrFI  (2513)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
BsaI  (2494)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BmrI  (2473)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
AhdI  (2433)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (1956)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PspFI  (1848)
1 site
C C C A G C G G G T C G
BseYI  (1844)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
AvaI  (1)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (1)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
PaeR7I  (1)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (1)
1 site
V C T C G A G B B G A G C T C V
XhoI  (1)
1 site
C T C G A G G A G C T C
BmeT110I  (2)
1 site
C Y C G R G G R G C Y C
HindIII  (253)
1 site
A A G C T T T T C G A A
KasI  (274)
1 site
G G C G C C C C G C G G
NarI  (275)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (276)
1 site
G G C G C C C C G C G G
PluTI  (278)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
Eco53kI  (292)
1 site
G A G C T C C T C G A G
SacI  (294)
1 site
G A G C T C C T C G A G
SalI  (383)
1 site
G T C G A C C A G C T G
AccI  (384)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
PspOMI  (393)
1 site
G G G C C C C C C G G G
ApaI  (397)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
BglII  (399)
1 site
A G A T C T T C T A G A
BspDI  (406)
1 site
A