Plasmid Files

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AfeI  (14) 1 site

BsgI  (3930) 1 site

Efficient cleavage requires at least two copies of the BsgI recognition sequence. Sticky ends from different BsgI sites may not be compatible. For full activity, add fresh S-adenosylmethionine (SAM).

AarI  (3902) 1 site

Efficient cleavage requires at least two copies of the AarI recognition sequence. Sticky ends from different AarI sites may not be compatible. After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.

ScaI  (3837) 1 site

TatI  (3835) 1 site

ApaLI  (3459) 1 site

BsaI  (2844) 1 site

Sticky ends from different BsaI sites may not be compatible. BsaI can be used between 37°C and 50°C.

PfoI  (2630) 1 site

Sticky ends from different PfoI sites may not be compatible.

RsrII  (2371) 1 site

Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible. For full activity, add fresh DTT.

BsrDI  (2088) 1 site

Sticky ends from different BsrDI sites may not be compatible.

PflFI  (1973) 1 site

The 1-base overhangs produced by PflFI may be hard to ligate. Sticky ends from different PflFI sites may not be compatible.

Tth111I  (1973) 1 site

The 1-base overhangs produced by Tth111I may be hard to ligate. Sticky ends from different Tth111I sites may not be compatible.

BglII  (27) 1 site

Eco53kI  (36) 1 site

SacI  (38) 1 site

Acc65I  (63) 1 site

KpnI  (67) 1 site

SacII  (70) 1 site

Efficient cleavage requires at least two copies of the SacII recognition sequence.

MluI  (76) 1 site

NheI  (82) 1 site

BmtI  (86) 1 site

AseI  (311) 1 site

XmnI  (312) 1 site

AgeI  (462) 1 site

AgeI quickly loses activity at 37°C, but can be used at 25°C for long incubations.

SgrAI  (462) 1 site

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.

PspOMI  (468) 1 site

ApaI  (472) 1 site

ApaI can be used between 25°C and 37°C.

TspMI  (474) 1 site

XmaI  (474) 1 site

Efficient cleavage requires at least two copies of the XmaI recognition sequence. Full cleavage with XmaI may require a long incubation.

SmaI  (476) 1 site

SmaI can be used at 37°C for brief incubations.

BamHI  (480) 1 site

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.

SpeI  (486) 1 site

BclI  (492) 1 site

* Blocked by Dam methylation. BclI is typically used at 50-55°C, but is 50% active at 37°C.

NotI  (499) 1 site

MfeI  (605) 1 site

HpaI  (618) 1 site

BtsαI  (694) 1 site

Sticky ends from different BtsI sites may not be compatible.

AflII  (737) 1 site

The sticky ends produced by AflII are hard to ligate.

DraIII  (971) 1 site

Sticky ends from different DraIII sites may not be compatible.

SexAI  (1444) 1 site

* Blocked by Dcm methylation. Sticky ends from different SexAI sites may not be compatible.

BglI  (1630) 1 site

Sticky ends from different BglI sites may not be compatible.

SfiI  (1630) 1 site

Efficient cleavage requires at least two copies of the SfiI recognition sequence. Sticky ends from different SfiI sites may not be compatible.

BseRI  (1673) 1 site

Sticky ends from different BseRI sites may not be compatible. BseRI quickly loses activity at 37°C. Prolonged incubation with BseRI may lead to degradation of the DNA.

StuI  (1676) 1 site

KasI  (1854) 1 site

NarI  (1855) 1 site

Efficient cleavage requires at least two copies of the NarI recognition sequence.

SfoI  (1856) 1 site

PluTI  (1858) 1 site

Efficient cleavage requires at least two copies of the PluTI recognition sequence.

MscI  (1937) 1 site

FspI  (1957) 1 site

NeoR/KanR 1727 .. 2521  =  795 bp 264 amino acids  =  29.0 kDa Product: aminoglycoside phosphotransferase from Tn5 confers resistance to neomycin, kanamycin, and G418 (Geneticin®)

NeoR/KanR 1727 .. 2521  =  795 bp 264 amino acids  =  29.0 kDa Product: aminoglycoside phosphotransferase from Tn5 confers resistance to neomycin, kanamycin, and G418 (Geneticin®)

ori 3129 .. 3717  =  589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

ori 3129 .. 3717  =  589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

f1 ori 747 .. 1202  =  456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis

f1 ori 747 .. 1202  =  456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis

SV40 promoter 1335 .. 1692  =  358 bp SV40 enhancer and early promoter

SV40 promoter 1335 .. 1692  =  358 bp SV40 enhancer and early promoter

ZFHD1 binding sites 88 .. 297  =  210 bp    Segment 1:      88 .. 99  =  12 bp 12 binding sites for the composite human DNA-binding domain ZFHD1 (Pomerantz et al., 1995)

ZFHD1 binding sites 88 .. 297  =  210 bp    Segment 2:      100 .. 105  =  6 bp 12 binding sites for the composite human DNA-binding domain ZFHD1 (Pomerantz et al., 1995)

ZFHD1 binding sites 88 .. 297  =  210 bp    Segment 3:      106 .. 117  =  12 bp 12 binding sites for the composite human DNA-binding domain ZFHD1 (Pomerantz et al., 1995)

ZFHD1 binding sites 88 .. 297  =  210 bp    Segment 4:      118 .. 123  =  6 bp 12 binding sites for the composite human DNA-binding domain ZFHD1 (Pomerantz et al., 1995)

ZFHD1 binding sites 88 .. 297  =  210 bp    Segment 5:      124 .. 135  =  12 bp 12 binding sites for the composite human DNA-binding domain ZFHD1 (Pomerantz et al., 1995)

ZFHD1 binding sites 88 .. 297  =  210 bp    Segment 6:      136 .. 141  =  6 bp 12 binding sites for the composite human DNA-binding domain ZFHD1 (Pomerantz et al., 1995)

ZFHD1 binding sites 88 .. 297  =  210 bp    Segment 7:      142 .. 153  =  12 bp 12 binding sites for the composite human DNA-binding domain ZFHD1 (Pomerantz et al., 1995)

ZFHD1 binding sites 88 .. 297  =  210 bp    Segment 8:      154 .. 159  =  6 bp 12 binding sites for the composite human DNA-binding domain ZFHD1 (Pomerantz et al., 1995)

ZFHD1 binding sites 88 .. 297  =  210 bp    Segment 9:      160 .. 171  =  12 bp 12 binding sites for the composite human DNA-binding domain ZFHD1 (Pomerantz et al., 1995)

ZFHD1 binding sites 88 .. 297  =  210 bp    Segment 10:      172 .. 177  =  6 bp 12 binding sites for the composite human DNA-binding domain ZFHD1 (Pomerantz et al., 1995)

ZFHD1 binding sites 88 .. 297  =  210 bp    Segment 11:      178 .. 189  =  12 bp 12 binding sites for the composite human DNA-binding domain ZFHD1 (Pomerantz et al., 1995)

ZFHD1 binding sites 88 .. 297  =  210 bp    Segment 12:      190 .. 195  =  6 bp 12 binding sites for the composite human DNA-binding domain ZFHD1 (Pomerantz et al., 1995)

ZFHD1 binding sites 88 .. 297  =  210 bp    Segment 13:      196 .. 207  =  12 bp 12 binding sites for the composite human DNA-binding domain ZFHD1 (Pomerantz et al., 1995)

ZFHD1 binding sites 88 .. 297  =  210 bp    Segment 14:      208 .. 213  =  6 bp 12 binding sites for the composite human DNA-binding domain ZFHD1 (Pomerantz et al., 1995)

ZFHD1 binding sites 88 .. 297  =  210 bp    Segment 15:      214 .. 225  =  12 bp 12 binding sites for the composite human DNA-binding domain ZFHD1 (Pomerantz et al., 1995)

ZFHD1 binding sites 88 .. 297  =  210 bp    Segment 16:      226 .. 231  =  6 bp 12 binding sites for the composite human DNA-binding domain ZFHD1 (Pomerantz et al., 1995)

ZFHD1 binding sites 88 .. 297  =  210 bp    Segment 17:      232 .. 243  =  12 bp 12 binding sites for the composite human DNA-binding domain ZFHD1 (Pomerantz et al., 1995)

ZFHD1 binding sites 88 .. 297  =  210 bp    Segment 18:      244 .. 249  =  6 bp 12 binding sites for the composite human DNA-binding domain ZFHD1 (Pomerantz et al., 1995)

ZFHD1 binding sites 88 .. 297  =  210 bp    Segment 19:      250 .. 261  =  12 bp 12 binding sites for the composite human DNA-binding domain ZFHD1 (Pomerantz et al., 1995)

ZFHD1 binding sites 88 .. 297  =  210 bp    Segment 20:      262 .. 267  =  6 bp 12 binding sites for the composite human DNA-binding domain ZFHD1 (Pomerantz et al., 1995)

ZFHD1 binding sites 88 .. 297  =  210 bp    Segment 21:      268 .. 279  =  12 bp 12 binding sites for the composite human DNA-binding domain ZFHD1 (Pomerantz et al., 1995)

ZFHD1 binding sites 88 .. 297  =  210 bp    Segment 22:      280 .. 285  =  6 bp 12 binding sites for the composite human DNA-binding domain ZFHD1 (Pomerantz et al., 1995)

ZFHD1 binding sites 88 .. 297  =  210 bp    Segment 23:      286 .. 297  =  12 bp 12 binding sites for the composite human DNA-binding domain ZFHD1 (Pomerantz et al., 1995)

ZFHD1 binding sites 88 .. 297  =  210 bp 23 segments 12 binding sites for the composite human DNA-binding domain ZFHD1 (Pomerantz et al., 1995)

SV40 poly(A) signal 619 .. 740  =  122 bp SV40 polyadenylation signal

SV40 poly(A) signal 619 .. 740  =  122 bp SV40 polyadenylation signal

minimal IL-2 promoter 320 .. 433  =  114 bp minimal promoter from the human interleukin-2 gene

minimal IL-2 promoter 320 .. 433  =  114 bp minimal promoter from the human interleukin-2 gene

AmpR promoter 1229 .. 1333  =  105 bp

AmpR promoter 1229 .. 1333  =  105 bp

pause site 3841 .. 3932  =  92 bp RNA polymerase II transcriptional pause signal from the human α2 globin gene

pause site 3841 .. 3932  =  92 bp RNA polymerase II transcriptional pause signal from the human α2 globin gene

poly(A) signal 3779 .. 3827  =  49 bp synthetic polyadenylation signal

poly(A) signal 3779 .. 3827  =  49 bp synthetic polyadenylation signal

HSV TK poly(A) signal 2753 .. 2800  =  48 bp herpesvirus thymidine kinase polyadenylation signal

HSV TK poly(A) signal 2753 .. 2800  =  48 bp herpesvirus thymidine kinase polyadenylation signal

MCS 462 .. 505  =  44 bp multiple cloning site

MCS 462 .. 505  =  44 bp multiple cloning site

SV40 ori 1543 .. 1678  =  136 bp SV40 origin of replication

SV40 ori 1543 .. 1678  =  136 bp SV40 origin of replication

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