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pcDNA™6.2/V5-pL-DEST

Promoterless Gateway® destination vector for cloning of mammalian promoters and genes.

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pcDNA6.2 V5-pL-DEST.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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SgrDI (6577) ZraI (6576) BglI (5776) PciI (4763) BspQI - SapI (4647) PfoI (4187) BlpI (4091) NruI * (3927) BsgI (3883) PpuMI (3855) EagI (3812) BbsI (3781) BclI * (3618) AvrII (3567) StuI (3566) AatII (6578) PstI (667) BfuAI - BspMI (670) BstXI (805) BmgBI (884) SrfI (918) BbvCI (1061) BssHII (1291) BamHI (1332) EcoRI (1787) BspEI (1791) XbaI * (2414) PspOMI (2420) ApaI (2424) SacII (2427) BstBI (2431) MluI (2475) AgeI (2481) PmeI (2499) SexAI * (3334) pcDNA™6.2/V5-pL-DEST 6693 bp
SgrDI  (6577)
1 site
C G T C G A C G G C A G C T G C
ZraI  (6576)
1 site
G A C G T C C T G C A G
BglI  (5776)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
PciI  (4763)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (4647)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (4647)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
PfoI  (4187)
1 site
T C C