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Plasmid Files

phCMV2

Compact mammalian cell vector for high-level expression of N-terminally HA-tagged proteins.

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phCMV2.dna
Map and Sequence File:    Download    Open   
Sequence Author:  MoBiTec
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AflIII - PciI (4203) ApaLI (3889) EcoO109I (3383) BsaI (3274) PfoI (3060) RsrII (2801) BsrDI (2518) PflFI - Tth111I (2403) FspI (2387) MscI (2367) PluTI (2288) SfoI (2286) NarI (2285) KasI (2284) AseI (7) NdeI (234) SnaBI (340) BsmBI (589) BbsI (634) BspEI (834) NheI (844) BmtI (848) BglII (855) PaeR7I - XhoI (859) HindIII (868) EcoRI (875) Acc65I (891) KpnI (895) PspOMI (899) TspMI - XmaI (902) ApaI (903) SmaI (904) BamHI (906) NotI (929) XbaI * (939) MfeI (1035) HpaI (1048) Bts α I (1124) AflII (1167) DraIII (1401) SexAI * (1874) SfiI (2060) BseRI (2103) StuI (2106) BspDI * - ClaI * (2125) phCMV2 4261 bp
AflIII  (4203)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (4203)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
ApaLI  (3889)
1 site
G T G C A C C A C G T G
EcoO109I  (3383)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BsaI  (3274)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
PfoI  (3060)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
RsrII  (2801)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2518)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2403)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2403)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FspI  (2387)
1 site
T G C G C A A C G C G T
MscI  (2367)
1 site
T G G C C A A C C G G T
PluTI  (2288)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (2286)
1 site
G G C G C C C C G C G G
NarI  (2285)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (2284)
1 site
G G C G C C C C G C G G
AseI  (7)
1 site
A T T A A T T A A T T A
NdeI  (234)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (340)
1 site
T A C G T A A T G C A T
BsmBI  (589)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BbsI  (634)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BspEI  (834)
1 site
T C C G G A A G G C C T
NheI  (844)
1 site
G C T A G C C G A T C G
BmtI  (848)
1 site
G C T A G C C G A T C G
BglII  (855)
1 site
A G A T C T T C T A G A
PaeR7I  (859)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (859)
1 site
C T C G A G G A G C T C
HindIII  (868)
1 site
A A G C T T T T C G A A
EcoRI  (875)
1 site
G A A T T C C T T A A G
Acc65I  (891)
1 site
G G T A C C C C A T G G
KpnI  (895)
1 site
G G T A C C C C A T G G
PspOMI  (899)
1 site
G G G C C C C C C G G G
TspMI  (902)
1 site
C C C G G G G G G C C C
XmaI  (902)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
ApaI  (903)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
SmaI  (904)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
BamHI  (906)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
NotI  (929)
1 site
G C G G C C G C C G C C G G C G
XbaI  (939)
1 site
T C T A G A A G A T C T
* Blocked by Dam methylation.
MfeI  (1035)
1 site
C A A T T G G T T A A C
HpaI  (1048)
1 site
G T T A A C C A A T T G
BtsαI  (1124)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsαI sites may not be compatible.
AflII  (1167)
1 site
C T T A A G G A A T T C
DraIII  (1401)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
SexAI  (1874)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
SfiI  (2060)
1 site
G G C C N N N N N G G C C