Plasmid Files

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.



EagI  (166) 1 site

NotI  (166) 1 site

PaeR7I  (158) 1 site

PaeR7I does not recognize the sequence CTCTCGAG.

PspXI  (158) 1 site

XhoI  (158) 1 site

BlpI  (80) 1 site

Sticky ends from different BlpI sites may not be compatible.

DraIII  (5180) 1 site

Sticky ends from different DraIII sites may not be compatible.

PsiI  (5052) 1 site

AsiSI  (4479) 1 site

PvuI  (4479) 1 site

SmaI  (4353) 1 site

SmaI can be used at 37°C for brief incubations.

TspMI  (4351) 1 site

XmaI  (4351) 1 site

Efficient cleavage requires at least two copies of the XmaI recognition sequence. Full cleavage with XmaI may require a long incubation.

NruI  (4136) 1 site

AcuI  (3825) 1 site

Efficient cleavage requires at least two copies of the AcuI recognition sequence. Sticky ends from different AcuI sites may not be compatible. After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility. For full activity, add fresh S-adenosylmethionine (SAM).

AlwNI  (3693) 1 site

Sticky ends from different AlwNI sites may not be compatible.

BssSαI  (3450) 1 site

PciI  (3277) 1 site

PciI is inhibited by nonionic detergents.

BspQI  (3161) 1 site

Sticky ends from different BspQI sites may not be compatible.

SapI  (3161) 1 site

Sticky ends from different SapI sites may not be compatible. SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.

TatI  (3081) 1 site

BstZ17I  (3048) 1 site

PflFI  (3022) 1 site

The 1-base overhangs produced by PflFI may be hard to ligate. Sticky ends from different PflFI sites may not be compatible.

Tth111I  (3022) 1 site

The 1-base overhangs produced by Tth111I may be hard to ligate. Sticky ends from different Tth111I sites may not be compatible.

HindIII  (173) 1 site

SalI  (179) 1 site

Eco53kI  (188) 1 site

SacI  (190) 1 site

EcoRI  (192) 1 site

BamHI  (198) 1 site

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.

EcoRV  (206) 1 site

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.

NcoI  (212) 1 site

Acc65I  (234) 1 site

KpnI  (238) 1 site

BglII  (241) 1 site

BstBI  (268) 1 site

NdeI  (346) 1 site

Prolonged incubation with NdeI may lead to removal of additional nucleotides.

XbaI  (384) 1 site

SgrAI  (495) 1 site

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.

SphI  (651) 1 site

BstAPI  (859) 1 site

Sticky ends from different BstAPI sites may not be compatible.

MluI  (1176) 1 site

BclI  (1190) 1 site

* Blocked by Dam methylation. BclI is typically used at 50-55°C, but is 50% active at 37°C.

BstEII  (1357) 1 site

Sticky ends from different BstEII sites may not be compatible. BstEII is typically used at 60°C, but is 50% active at 37°C.

NmeAIII  (1382) 1 site

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence. Sticky ends from different NmeAIII sites may not be compatible. For full activity, add fresh S-adenosylmethionine (SAM).

PspOMI  (1383) 1 site

ApaI  (1387) 1 site

ApaI can be used between 25°C and 37°C.

BssHII  (1587) 1 site

BssHII is typically used at 50°C, but is 75% active at 37°C.

HpaI  (1682) 1 site

PshAI  (2021) 1 site

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.

BglI  (2240) 1 site

Sticky ends from different BglI sites may not be compatible.

FspI  (2258) 1 site

FspAI  (2258) 1 site

PpuMI  (2283) 1 site

Sticky ends from different PpuMI sites may not be compatible.

lacI 826 .. 1908  =  1083 bp 360 amino acids  =  38.6 kDa Product: lac repressor The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).

lacI 826 .. 1908  =  1083 bp 360 amino acids  =  38.6 kDa Product: lac repressor The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).

KanR 4048 .. 4863  =  816 bp 271 amino acids  =  31.0 kDa Product: aminoglycoside phosphotransferase confers resistance to kanamycin

KanR 4048 .. 4863  =  816 bp 271 amino acids  =  31.0 kDa Product: aminoglycoside phosphotransferase confers resistance to kanamycin

ori 3338 .. 3926  =  589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

ori 3338 .. 3926  =  589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

f1 ori 4956 .. 5411  =  456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis

f1 ori 4956 .. 5411  =  456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis

rop 2717 .. 2908  =  192 bp 63 amino acids  =  7.2 kDa Product: Rop protein

rop 2717 .. 2908  =  192 bp 63 amino acids  =  7.2 kDa Product: Rop protein

lacI promoter 748 .. 825  =  78 bp

lacI promoter 748 .. 825  =  78 bp

MCS 158 .. 217  =  60 bp multiple cloning site

MCS 158 .. 217  =  60 bp multiple cloning site

T7 terminator 26 .. 73  =  48 bp transcription terminator for bacteriophage T7 RNA polymerase

T7 terminator 26 .. 73  =  48 bp transcription terminator for bacteriophage T7 RNA polymerase

S-Tag 249 .. 293  =  45 bp 15 amino acids  =  1.7 kDa Product: affinity and epitope tag derived from pancreatic ribonuclease A

S-Tag 249 .. 293  =  45 bp 15 amino acids  =  1.7 kDa Product: affinity and epitope tag derived from pancreatic ribonuclease A

lac operator 392 .. 416  =  25 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).

lac operator 392 .. 416  =  25 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).

T7 promoter 417 .. 435  =  19 bp promoter for bacteriophage T7 RNA polymerase

T7 promoter 417 .. 435  =  19 bp promoter for bacteriophage T7 RNA polymerase

6xHis 140 .. 157  =  18 bp 6 amino acids  =  840.9 Da Product: 6xHis affinity tag

6xHis 140 .. 157  =  18 bp 6 amino acids  =  840.9 Da Product: 6xHis affinity tag

thrombin site 300 .. 317  =  18 bp 6 amino acids  =  627.8 Da Product: thrombin recognition and cleavage site

thrombin site 300 .. 317  =  18 bp 6 amino acids  =  627.8 Da Product: thrombin recognition and cleavage site

6xHis 327 .. 344  =  18 bp 6 amino acids  =  840.9 Da Product: 6xHis affinity tag

6xHis 327 .. 344  =  18 bp 6 amino acids  =  840.9 Da Product: 6xHis affinity tag

enterokinase site 219 .. 233  =  15 bp 5 amino acids  =  606.5 Da Product: enterokinase recognition and cleavage site

enterokinase site 219 .. 233  =  15 bp 5 amino acids  =  606.5 Da Product: enterokinase recognition and cleavage site

RBS 356 .. 361  =  6 bp ribosome binding site

RBS 356 .. 361  =  6 bp ribosome binding site

ATG 345 .. 347  =  3 bp 1 amino acid  =  149.2 Da Product: start codon

ATG 345 .. 347  =  3 bp 1 amino acid  =  149.2 Da Product: start codon

Try SnapGene and create your own beautiful maps

SnapGene®	SnapGene® Viewer
SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures. Priced accessibly so that everyone in your lab can have a license. Learn More...	SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats. Free! Because there should be no barriers to seeing your data. Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

hidden link