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pET-32c(+)

Bacterial vector for expressing thioredoxin fusion proteins with an enterokinase site. For other reading frames, use pET‑32a(+) or pET‑32b(+).

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pET-32c(+).dna
Map and Sequence File:    Download    Open   
Sequence Author:  Novagen (EMD Millipore)
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DraIII (5659) PsiI (5531) ScaI (4996) PvuI (4886) PstI (4761) BsaI (4577) AhdI (4516) AlwNI (4039) PciI (3623) BspQI - SapI (3507) BstZ17I (3394) PflFI - Tth111I (3368) BlpI (80) AvaI - BsoBI - PaeR7I - PspXI - XhoI (158) BmeT110I (159) EagI - NotI (166) HindIII (173) SalI (179) Eco53kI (188) SacI (190) EcoRI (192) BamHI (198) EcoRV (209) NcoI (213) Acc65I (235) KpnI (239) BglII (242) BstBI (269) MscI (352) RsrII (590) XbaI (730) SgrAI (841) SphI (997) EcoNI (1057) BstAPI (1205) MluI (1522) BclI * (1536) BstEII (1703) PspOMI (1729) ApaI (1733) BssHII (1933) HpaI (2028) PshAI (2367) FspAI (2604) PpuMI (2629) Bpu10I (2729) pET-32c(+) 5901 bp
DraIII  (5659)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PsiI  (5531)
1 site
T T A T A A A A T A T T
ScaI  (4996)
1 site
A G T A C T T C A T G A
PvuI  (4886)
1 site
C G A T C G G C T A G C
PstI  (4761)
1 site
C T G C A G G A C G T C
BsaI  (4577)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (4516)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (4039)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PciI  (3623)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (3507)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (3507)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BstZ17I  (3394)
1 site
G T A T A C C A T A T G
PflFI  (3368)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3368)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BlpI  (80)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
AvaI  (158)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (158)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
PaeR7I  (158)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (158)
1 site
V C T C G A G B B G A G C T C V
XhoI  (158)
1 site
C T C G A G G A G C T C
BmeT110I  (159)
1 site
C Y C G R G G R G C Y C
EagI  (166)
1 site
C G G C C G G C C G G C
NotI  (166)
1 site
G C G G C C G C C G C C G G C G
HindIII  (173)
1 site
A A G C T T T T C G A A
SalI  (179)
1 site
G T C G A C C A G C T G
Eco53kI  (188)
1 site
G A G C T C C T C G A G
SacI  (190)
1 site
G A G C T C C T C G A G
EcoRI  (192)
1 site
G A A T T C C T T A A G
BamHI  (198)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
EcoRV  (209)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
NcoI  (213)
1 site
C C A T G G G G T A C C
Acc65I  (235)
1 site
G G T A C C C C A T G G
KpnI  (239)
1 site
G G T A C C C C A T