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pET-39b(+)

Bacterial vector for expressing periplasmic DsbA fusion proteins.

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pET-39b(+).dna
Map and Sequence File:    Download    Open   
Sequence Author:  MilliporeSigma (Novagen)
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DraIII (5864) PsiI (5736) AsiSI - PvuI (5163) NruI (4820) AcuI (4509) AlwNI (4377) BssS α I (4134) PciI (3961) BspQI - SapI (3845) BstZ17I (3732) PflFI - Tth111I (3706) AvrII (131) PaeR7I - PspXI - XhoI (174) EagI - NotI (182) HindIII (189) SalI (195) Eco53kI (204) SacI (206) EcoRI (208) BamHI (214) NcoI (227) SrfI (246) BseRI (250) Acc65I (267) KpnI (271) BstBI (301) ScaI (341) SacII (365) SpeI (396) AflII (418) PstI (552) BfuAI - BspMI (636) AscI (917) AgeI (928) NdeI (1030) XbaI (1068) SgrAI (1179) SphI (1335) BstAPI (1543) MluI (1860) BclI * (1874) NmeAIII (2066) PspOMI (2067) ApaI (2071) PshAI (2705) BglI (2924) FspI - FspAI (2942) PpuMI (2967) pET-39b(+) 6106 bp
DraIII  (5864)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PsiI  (5736)
1 site
T T A T A A A A T A T T
AsiSI  (5163)
1 site
G C G A T C G C C G C T A G C G
PvuI  (5163)
1 site
C G A T C G G C T A G C
NruI  (4820)
1 site
T C G C G A A G C G C T
AcuI  (4509)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the AcuI recognition sequence.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
AlwNI  (4377)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BssSαI  (4134)
1 site
C A C G A G G T G C T C
PciI  (3961)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (3845)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (3845)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BstZ17I  (3732)
1 site
G T A T A C C A T A T G
PflFI  (3706)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3706)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
AvrII  (131)
1 site
C C T A G G G G A T C C
PaeR7I  (174)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (174)
1 site
V C T C G A G B B G A G C T C V
XhoI  (174)
1 site
C T C G A G G A G C T C
EagI  (182)
1 site
C G G C C G G C C G G C
NotI  (182)
1 site
G C G G C C G C C G C C G G C G
HindIII  (189)
1 site
A A G C T T T T C G A A
SalI  (195)
1 site
G T C G A C C A G C T G
Eco53kI  (204)
1 site
G A G C T C C T C G A G
SacI  (206)
1 site
G A G C T C C T C G A G
EcoRI  (208)
1 site
G A A T T C C T T A A G
BamHI  (214)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
NcoI  (227)
1 site
C C A T G G G G T A C C
SrfI  (246)
1 site
G C C C G G G C C G G G C C C G
BseRI  (250)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
Acc65I  (267)
1 site
G G T A C C C C A T G G
KpnI  (271)
1 site
G G T A C C C C A T G G
BstBI  (301)
1 site
T T C G A A A A G C T T
ScaI  (341)
1 site
A G T A C T T C A T G A
SacII  (365)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
SpeI  (396)
1 site
A C T A G T T G A T C A
AflII  (418)
1 site
C T T A A G G A A T T C
PstI  (552)
1 site
C T G C A G G A C G T C
BfuAI  (636)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (636)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
AscI  (917)
1 site
G G C G C G C C C C G C G C G G
AgeI  (928)
1 site
A C C G G T T G G C C A
NdeI  (1030)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
XbaI  (1068)
1 site
T C T A G A A G A T C T
SgrAI  (1179)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
SphI  (1335)
1 site
G C A T G C C G T A C G
BstAPI  (1543)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
MluI  (1860)
1 site
A C G C G T T G C G C A
BclI  (1874)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
NmeAIII  (2066)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PspOMI  (2067)
1 site
G G G C C C C C C G G G
ApaI  (2071)
1 site
G G G C C