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Plasmid Files

pET-43.1b(+)

Bacterial vector for expressing NusA fusion proteins. For other reading frames, use pET‑43.1a(+) or pET‑43.1c(+).

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pET-43.1b(+).dna
Map and Sequence File:    Download    Open   
Sequence Author:  Novagen (EMD Millipore)
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DraIII (7032) PsiI (6904) AhdI (6620) BglI (6502) FspI (6397) ScaI (6139) AlwNI (5426) PciI (5010) BspQI - SapI (4894) PflFI - Tth111I (4755) Bpu10I (4116) PpuMI (4016) HpaI (3652) AvrII (483) PacI (503) PaeR7I - XhoI (530) PmlI (576) EagI - NotI (592) HindIII (599) Acc65I (620) KpnI (624) SalI - SgrDI (626) PstI - SbfI (639) AscI (641) BsrGI (648) EcoRI (654) BamHI (660) Eco53kI (670) SacI (672) PshAI (680) TspMI - XmaI (718) SmaI (720) SacII (796) SpeI (827) BglII (942) BsmI (986) MscI (1074) StuI (1127) BfuAI - BspMI (1276) NcoI (1402) NdeI (2316) XbaI (2354) SphI (2621) EcoNI (2681) BstEII (3327) PspOMI (3353) ApaI (3357) pET-43.1b(+) 7274 bp
DraIII  (7032)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PsiI  (6904)
1 site
T T A T A A A A T A T T
AhdI  (6620)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BglI  (6502)
1 site
G C C N N N N N G G C C G G N N N N