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Plasmid Files

pCAMBIA1200

Agrobacterium binary vector for plant transformation, with hygromycin- and chloramphenicol-resistance genes.

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pCAMBIA1200 Sequence and MappCAMBIA1200.dna
Map and Sequence File   
Sequence Author:  Cambia
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 PmeI (8991) HindIII (8778) PstI - SbfI (8770) SalI (8760) XbaI (8754) BamHI (8748) KpnI (8743) Acc65I (8739) BanII - SacI (8737) Eco53kI (8735) EcoRI (8727) lac operator BstXI (8484) EcoRV (7832) AatII (7643) ZraI (7641) AsiSI (7304) RsrII (7255) PspXI (6603) BclI * (6088) SspI (5272) HpaI (5065) NsiI (4945) BsaBI * (1331) BsiWI (2892) NheI (3008) BmtI (3012) BspDI * - ClaI * (3088) EcoNI (3321) BsaI (3411) KasI (3793) NarI (3794) SfoI (3795) PluTI (3797) BstZ17I (3992) pCAMBIA1200 9111 bp
PmeI  (8991)
1 site
G T T T A A A C C A A A T T T G
HindIII  (8778)
1 site
A A G C T T T T C G A A
PstI  (8770)
1 site
C T G C A G G A C G T C
SbfI  (8770)
1 site
C C T G C A G G G G A C G T C C
SalI  (8760)
1 site
G T C G A C C A G C T G
XbaI  (8754)
1 site
T C T A G A A G A T C T
BamHI  (8748)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
KpnI  (8743)
1 site
G G T A C C C C A T G G
Acc65I  (8739)
1 site
G G T A C C C C A T G G
BanII  (8737)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
SacI  (8737)
1 site
G A G C T C C T C G A G
Eco53kI  (8735)
1 site
G A G C T C C T C G A G
EcoRI  (8727)
1 site
G A A T T C C T T A A G
BstXI  (8484)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
EcoRV  (7832)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to
delete a base after cleavage.
AatII  (7643)
1 site
G A C G T C C T G C A G
ZraI  (7641)
1 site
G A C G T C C T G C A G
AsiSI  (7304)
1 site
G C G A T C G C C G C T A G C G
RsrII  (7255)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII
recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
PspXI  (6603)
1 site
V C T C G A G B B G A G C T C V
BclI  (6088)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
SspI  (5272)
1 site
A A T A T T T T A T A A
HpaI  (5065)
1 site
G T T A A C C A A T T G
NsiI  (4945)
1 site
A T G C A T T A C G T A
BsaBI  (1331)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
BsiWI  (2892)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
NheI  (3008)
1 site
G C T A G C C G A T C G
BmtI  (3012)
1 site
G C T A G C C G A T C G
BspDI  (3088)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (3088)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
EcoNI  (3321)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
BsaI  (3411)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
KasI  (3793)
1 site
G G C G C C C C G C G G
NarI  (3794)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
SfoI  (3795)
1 site
G G C G C C C C G C G G
PluTI  (3797)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
BstZ17I  (3992)
1 site
G T A T A C C A T A T G
pVS1 RepA
2277 .. 3350  =  1074 bp
357 amino acids  =  39.9 kDa
Product: replication protein from Pseudomonas
plasmid pVS1
pVS1 RepA
2277 .. 3350  =  1074 bp
357 amino acids  =  39.9 kDa
Product: replication protein from Pseudomonas
plasmid pVS1
HygR
6642 .. 7667  =  1026 bp
341 amino acids  =  38.0 kDa
Product: hygromycin B phosphotransferase
confers resistance to hygromycin
HygR
6642 .. 7667  =  1026 bp
341 amino acids  =  38.0 kDa
Product: hygromycin B phosphotransferase
confers resistance to hygromycin
CaMV 35S promoter (enhanced)
7735 .. 8412  =  678 bp
cauliflower mosaic virus 35S promoter with a
duplicated enhancer region
CaMV 35S promoter (enhanced)
7735 .. 8412  =  678 bp
cauliflower mosaic virus 35S promoter with a
duplicated enhancer region
CmR
5139 .. 5798  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
5139 .. 5798  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
pVS1 StaA
1219 .. 1848  =  630 bp
209 amino acids  =  22.1 kDa
Product: stability protein from Pseudomonas
plasmid pVS1
pVS1 StaA
1219 .. 1848  =  630 bp
209 amino acids  =  22.1 kDa
Product: stability protein from Pseudomonas
plasmid pVS1
ori
4280 .. 4868  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
4280 .. 4868  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
lacZα
8713 .. 8946  =  234 bp
77 amino acids  =  8.6 kDa
Product: LacZα fragment of β-galactosidase
lacZα
8713 .. 8946  =  234 bp
77 amino acids  =  8.6 kDa
Product: LacZα fragment of β-galactosidase
pVS1 oriV
3416 .. 3610  =  195 bp
origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)
pVS1 oriV
3416 .. 3610  =  195 bp
origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)
CaMV poly(A) signal
6428 .. 6602  =  175 bp
cauliflower mosaic virus polyadenylation signal
CaMV poly(A) signal
6428 .. 6602  =  175 bp
cauliflower mosaic virus polyadenylation signal
bom
3954 .. 4094  =  141 bp
basis of mobility region from pBR322
bom
3954 .. 4094  =  141 bp
basis of mobility region from pBR322
lac promoter
8639 .. 8669  =  31 bp
   Segment 1:  -35  
   8639 .. 8644  =  6 bp
promoter for the E. coli lac operon
lac promoter
8639 .. 8669  =  31 bp
   Segment 2:  
   8645 .. 8662  =  18 bp
promoter for the E. coli lac operon
lac promoter
8639 .. 8669  =  31 bp
   Segment 3:  -10  
   8663 .. 8669  =  7 bp
promoter for the E. coli lac operon
lac promoter
8639 .. 8669  =  31 bp
3 segments
promoter for the E. coli lac operon
LB T-DNA repeat
6326 .. 6350  =  25 bp
left border repeat from nopaline C58 T-DNA
LB T-DNA repeat
6326 .. 6350  =  25 bp
left border repeat from nopaline C58 T-DNA
RB T-DNA repeat
9006 .. 9030  =  25 bp
right border repeat from nopaline C58 T-DNA
RB T-DNA repeat
9006 .. 9030  =  25 bp
right border repeat from nopaline C58 T-DNA
lac operator
8677 .. 8693  =  17 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
8677 .. 8693  =  17 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
MCS
8727 .. 8783  =  57 bp
pUC18/19 multiple cloning site
MCS
8727 .. 8783  =  57 bp
pUC18/19 multiple cloning site
M13 rev
8701 .. 8717  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 rev
8701 .. 8717  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 fwd
8787 .. 8803  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 fwd
8787 .. 8803  =  17 bp
common sequencing primer, one of multiple similar
variants
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