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pCAMBIA3200

Agrobacterium binary vector for plant transformation, with bialophos/phosphinothricin resistance and chloramphenicol resistance genes.

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pCAMBIA3200.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Cambia
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PmeI (8461) PvuI (8374) HindIII (8248) PstI - SbfI (8240) XbaI (8224) BamHI (8218) SmaI (8215) TspMI - XmaI (8213) SacI (8207) Eco53kI (8205) EcoRI (8197) BstXI (7954) EcoRV (7302) AfeI (6994) ApaI (6938) EcoO109I - PspOMI (6934) AarI (6821) FspAI (6757) SacII (6727) PspXI (6603) BclI * (6088) BspEI (5584) SspI (5272) HpaI (5065) NsiI (4945) BsaBI * (1331) BsiWI (2892) NheI (3008) BmtI (3012) BspDI * - ClaI * (3088) EcoNI (3321) BsaI (3411) AgeI (3502) KasI (3793) NarI (3794) SfoI (3795) PluTI (3797) BstZ17I (3992) pCAMBIA3200 8581 bp
PmeI  (8461)
1 site
G T T T A A A C C A A A T T T G
PvuI  (8374)
1 site
C G A T C G G C T A G C
HindIII  (8248)
1 site
A A G C T T T T C G A A
PstI  (8240)
1 site
C T G C A G G A C G T C
SbfI  (8240)
1 site
C C T G C A G G G G A C G T C C
XbaI  (8224)
1 site
T C T A G A A G A T C T
BamHI  (8218)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SmaI  (8215)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (8213)
1 site
C C C G G G G G G C C C
XmaI  (8213)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
SacI  (8207)
1 site
G A G C T C C T C G A G
Eco53kI  (8205)
1 site
G A G C T C C T C G A G
EcoRI  (8197)
1 site
G A A T T C C T T A A G
BstXI  (7954)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
EcoRV  (7302)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
AfeI  (6994)
1 site
A G C G C T T C G C G A
ApaI  (6938)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
EcoO109I  (6934)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PspOMI  (6934)
1 site
G G G C C C C C C G