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Plasmid Files

pCAMBIA5105

Plant vector containing vir operons from pTiBo542, compatible with a range of non-Agrobacterium bacteria.

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pCAMBIA5105 Sequence and MappCAMBIA5105.dna
Map and Sequence File   
Sequence Author:  Cambia
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 CaMV poly(A) signal lac promoter lac operator SwaI (47,077) AvrII (47,068) SbfI (47,065) CaMV 35S promoter SpeI (46,076) 6xHis RB T-DNA repeat pVS1 oriV bom M13 ori M13 fwd PmeI (29,070) LB T-DNA repeat pCAMBIA5105 49,757 bp
SwaI  (47,077)
1 site
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
AvrII  (47,068)
1 site
C C T A G G G G A T C C
SbfI  (47,065)
1 site
C C T G C A G G G G A C G T C C
SpeI  (46,076)
1 site
A C T A G T T G A T C A
PmeI  (29,070)
1 site
G T T T A A A C C A A A T T T G
virD5
26,569 .. 29,079  =  2511 bp
836 amino acids  =  93.3 kDa
Product: VirD5
virD5
26,569 .. 29,079  =  2511 bp
836 amino acids  =  93.3 kDa
Product: VirD5
virA
2014 .. 4503  =  2490 bp
829 amino acids  =  91.5 kDa
Product: VirA
sensory component of two-component signal
transduction system
virA
2014 .. 4503  =  2490 bp
829 amino acids  =  91.5 kDa
Product: VirA
sensory component of two-component signal
transduction system
virB4
11,473 .. 13,842  =  2370 bp
789 amino acids  =  87.4 kDa
Product: VirB4
integral inner membrane ATPase
virB4
11,473 .. 13,842  =  2370 bp
789 amino acids  =  87.4 kDa
Product: VirB4
integral inner membrane ATPase
GUSPlus™
44,273 .. 46,295  =  2023 bp
611 amino acids  =  69.8 kDa
2 segments
Product: β-glucuronidase
codon-optimized Staphylococcus gusA gene with a
catalase intron to ensure expression in plants but
not bacteria
GUSPlus™
44,273 .. 46,295  =  2023 bp
611 amino acids  =  69.8 kDa
   Segment 2:  
   44,273 .. 46,090  =  1818 bp
   606 amino acids  =  69.2 kDa
Product: β-glucuronidase
codon-optimized Staphylococcus gusA gene with a
catalase intron to ensure expression in plants but
not bacteria
GUSPlus™
44,273 .. 46,295  =  2023 bp
611 amino acids  =  69.8 kDa
   Segment 1:  
   46,281 .. 46,295  =  15 bp
   5 amino acids  =  632.8 Da
Product: β-glucuronidase
codon-optimized Staphylococcus gusA gene with a
catalase intron to ensure expression in plants but
not bacteria
GUSPlus™
44,273 .. 46,295  =  2023 bp
611 amino acids  =  69.8 kDa
2 segments
Product: β-glucuronidase
codon-optimized Staphylococcus gusA gene with a
catalase intron to ensure expression in plants but
not bacteria
virE3
31,447 .. 33,465  =  2019 bp
672 amino acids  =  75.7 kDa
Product: VirE3
virE3
31,447 .. 33,465  =  2019 bp
672 amino acids  =  75.7 kDa
Product: VirE3
virD4
24,510 .. 26,468  =  1959 bp
652 amino acids  =  72.1 kDa
Product: VirD4
coupling protein
virD4
24,510 .. 26,468  =  1959 bp
652 amino acids  =  72.1 kDa
Product: VirD4
coupling protein
virE2
29,731 .. 31,380  =  1650 bp
549 amino acids  =  62.6 kDa
Product: VirE2
single-stranded DNA binding protein
virE2
29,731 .. 31,380  =  1650 bp
549 amino acids  =  62.6 kDa
Product: VirE2
single-stranded DNA binding protein
virD2
22,581 .. 23,855  =  1275 bp
424 amino acids  =  47.5 kDa
Product: VirD2
relaxase
virD2
22,581 .. 23,855  =  1275 bp
424 amino acids  =  47.5 kDa
Product: VirD2
relaxase
virB10
17,279 .. 18,412  =  1134 bp
377 amino acids  =  40.5 kDa
Product: VirB10
T-DNA transfer
virB10
17,279 .. 18,412  =  1134 bp
377 amino acids  =  40.5 kDa
Product: VirB10
T-DNA transfer
pVS1 RepA
40,488 .. 41,561  =  1074 bp
357 amino acids  =  39.9 kDa
Product: replication protein from Pseudomonas
plasmid pVS1
pVS1 RepA
40,488 .. 41,561  =  1074 bp
357 amino acids  =  39.9 kDa
Product: replication protein from Pseudomonas
plasmid pVS1
virB11
18,452 .. 19,483  =  1032 bp
343 amino acids  =  38.0 kDa
Product: VirB11
T-DNA transfer
virB11
18,452 .. 19,483  =  1032 bp
343 amino acids  =  38.0 kDa
Product: VirB11
T-DNA transfer
HygR
48,176 .. 49,201  =  1026 bp
341 amino acids  =  38.0 kDa
Product: hygromycin B phosphotransferase
confers resistance to hygromycin
HygR
48,176 .. 49,201  =  1026 bp
341 amino acids  =  38.0 kDa
Product: hygromycin B phosphotransferase
confers resistance to hygromycin
virB6
14,616 .. 15,503  =  888 bp
295 amino acids  =  31.8 kDa
Product: VirB6
T-DNA transfer
virB6
14,616 .. 15,503  =  888 bp
295 amino acids  =  31.8 kDa
Product: VirB6
T-DNA transfer
virG
19,615 .. 20,418  =  804 bp
267 amino acids  =  30.0 kDa
Product: VirG
response regulator of virA/virG two component
transduction system
virG
19,615 .. 20,418  =  804 bp
267 amino acids  =  30.0 kDa
Product: VirG
response regulator of virA/virG two component
transduction system
NeoR/KanR
36,190 .. 36,984  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin®)
NeoR/KanR
36,190 .. 36,984  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin®)
SmR
37,935 .. 38,726  =  792 bp
263 amino acids  =  29.2 kDa
Product: aminoglycoside adenylyltransferase
confers resistance to spectinomycin and
streptomycin
SmR
37,935 .. 38,726  =  792 bp
263 amino acids  =  29.2 kDa
Product: aminoglycoside adenylyltransferase
confers resistance to spectinomycin and
streptomycin
virJ
7828 .. 8571  =  744 bp
247 amino acids  =  27.2 kDa
Product: VirJ
virJ
7828 .. 8571  =  744 bp
247 amino acids  =  27.2 kDa
Product: VirJ
virB1
10,048 .. 10,767  =  720 bp
239 amino acids  =  25.9 kDa
Product: VirB1
T-DNA transfer
virB1
10,048 .. 10,767  =  720 bp
239 amino acids  =  25.9 kDa
Product: VirB1
T-DNA transfer
virB8
15,691 .. 16,404  =  714 bp
237 amino acids  =  26.2 kDa
Product: VirB8
T-DNA transfer
virB8
15,691 .. 16,404  =  714 bp
237 amino acids  =  26.2 kDa
Product: VirB8
T-DNA transfer
virC1
21,140 .. 21,835  =  696 bp
231 amino acids  =  25.6 kDa
Product: VirC1
virC1
21,140 .. 21,835  =  696 bp
231 amino acids  =  25.6 kDa
Product: VirC1
CaMV 35S promoter (enhanced)
47,431 .. 48,108  =  678 bp
cauliflower mosaic virus 35S promoter with a
duplicated enhancer region
CaMV 35S promoter (enhanced)
47,431 .. 48,108  =  678 bp
cauliflower mosaic virus 35S promoter with a
duplicated enhancer region
virB5
13,857 .. 14,519  =  663 bp
220 amino acids  =  23.5 kDa
Product: VirB5
T-DNA transfer
virB5
13,857 .. 14,519  =  663 bp
220 amino acids  =  23.5 kDa
Product: VirB5
T-DNA transfer
pVS1 StaA
41,990 .. 42,619  =  630 bp
209 amino acids  =  22.1 kDa
Product: stability protein from Pseudomonas
plasmid pVS1
pVS1 StaA
41,990 .. 42,619  =  630 bp
209 amino acids  =  22.1 kDa
Product: stability protein from Pseudomonas
plasmid pVS1
virC2
20,529 .. 21,137  =  609 bp
202 amino acids  =  22.4 kDa
Product: VirC2
virC2
20,529 .. 21,137  =  609 bp
202 amino acids  =  22.4 kDa
Product: VirC2
ori
38,970 .. 39,558  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
38,970 .. 39,558  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
virD1
22,104 .. 22,547  =  444 bp
147 amino acids  =  16.3 kDa
Product: VirD1
cleavage accessory protein
virD1
22,104 .. 22,547  =  444 bp
147 amino acids  =  16.3 kDa
Product: VirD1
cleavage accessory protein
virK
262 .. 699  =  438 bp
145 amino acids  =  15.8 kDa
Product: VirK
virK
262 .. 699  =  438 bp
145 amino acids  =  15.8 kDa
Product: VirK
M13 ori
35,442 .. 35,822  =  381 bp
M13 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
M13 ori
35,442 .. 35,822  =  381 bp
M13 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
virB2
10,782 .. 11,147  =  366 bp
121 amino acids  =  12.3 kDa
Product: VirB2
pilin protein precursor
virB2
10,782 .. 11,147  =  366 bp
121 amino acids  =  12.3 kDa
Product: VirB2
pilin protein precursor
CaMV 35S promoter
46,312 .. 46,657  =  346 bp
strong constitutive promoter from cauliflower
mosaic virus
CaMV 35S promoter
46,312 .. 46,657  =  346 bp
strong constitutive promoter from cauliflower
mosaic virus
NOS terminator
43,965 .. 44,217  =  253 bp
nopaline synthase terminator and poly(A) signal
NOS terminator
43,965 .. 44,217  =  253 bp
nopaline synthase terminator and poly(A) signal
lacZα
46,891 .. 47,130  =  240 bp
79 amino acids  =  8.7 kDa
Product: LacZα fragment of β-galactosidase
lacZα
46,891 .. 47,130  =  240 bp
79 amino acids  =  8.7 kDa
Product: LacZα fragment of β-galactosidase
virE1
29,529 .. 29,726  =  198 bp
65 amino acids  =  7.3 kDa
Product: VirE1
chaperone for VirE2
virE1
29,529 .. 29,726  =  198 bp
65 amino acids  =  7.3 kDa
Product: VirE1
chaperone for VirE2
pVS1 oriV
40,228 .. 40,422  =  195 bp
origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)
pVS1 oriV
40,228 .. 40,422  =  195 bp
origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)
CaMV poly(A) signal
49,241 .. 49,415  =  175 bp
cauliflower mosaic virus polyadenylation signal
CaMV poly(A) signal
49,241 .. 49,415  =  175 bp
cauliflower mosaic virus polyadenylation signal
bom
39,744 .. 39,884  =  141 bp
basis of mobility region from pBR322
bom
39,744 .. 39,884  =  141 bp
basis of mobility region from pBR322
lac promoter
47,174 .. 47,204  =  31 bp
   Segment 3:  -10  
   47,174 .. 47,180  =  7 bp
promoter for the E. coli lac operon
lac promoter
47,174 .. 47,204  =  31 bp
   Segment 2:  
   47,181 .. 47,198  =  18 bp
promoter for the E. coli lac operon
lac promoter
47,174 .. 47,204  =  31 bp
   Segment 1:  -35  
   47,199 .. 47,204  =  6 bp
promoter for the E. coli lac operon
lac promoter
47,174 .. 47,204  =  31 bp
3 segments
promoter for the E. coli lac operon
RB T-DNA repeat
43,919 .. 43,943  =  25 bp
right border repeat from nopaline C58 T-DNA
RB T-DNA repeat
43,919 .. 43,943  =  25 bp
right border repeat from nopaline C58 T-DNA
LB T-DNA repeat
49,493 .. 49,517  =  25 bp
left border repeat from nopaline C58 T-DNA
LB T-DNA repeat
49,493 .. 49,517  =  25 bp
left border repeat from nopaline C58 T-DNA
T7 promoter
35,260 .. 35,278  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
35,260 .. 35,278  =  19 bp
promoter for bacteriophage T7 RNA polymerase
6xHis
44,249 .. 44,266  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
44,249 .. 44,266  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
M13 fwd
35,285 .. 35,301  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 fwd
35,285 .. 35,301  =  17 bp
common sequencing primer, one of multiple similar
variants
lac operator
47,150 .. 47,166  =  17 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
47,150 .. 47,166  =  17 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
virB9
16,401 .. 17,282  =  882 bp
293 amino acids  =  32.1 kDa
Product: VirB9
T-DNA transfer
virB9
16,401 .. 17,282  =  882 bp
293 amino acids  =  32.1 kDa
Product: VirB9
T-DNA transfer
virD3
23,875 .. 24,513  =  639 bp
212 amino acids  =  22.7 kDa
Product: VirD3
virD3
23,875 .. 24,513  =  639 bp
212 amino acids  =  22.7 kDa
Product: VirD3
virB3
11,147 .. 11,473  =  327 bp
108 amino acids  =  11.8 kDa
Product: VirB3
T-DNA transfer
virB3
11,147 .. 11,473  =  327 bp
108 amino acids  =  11.8 kDa
Product: VirB3
T-DNA transfer
cat1 intron
46,091 .. 46,280  =  190 bp
castor bean catalase intron, modified
cat1 intron
46,091 .. 46,280  =  190 bp
castor bean catalase intron, modified
virB7
15,537 .. 15,704  =  168 bp
55 amino acids  =  5.8 kDa
Product: VirB7
T-DNA transfer
virB7
15,537 .. 15,704  =  168 bp
55 amino acids  =  5.8 kDa
Product: VirB7
T-DNA transfer
M13 fwd
47,034 .. 47,050  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 fwd
47,034 .. 47,050  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 rev
47,126 .. 47,142  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 rev
47,126 .. 47,142  =  17 bp
common sequencing primer, one of multiple similar
variants
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