Resources
Plasmid Files

pSB1

Superbinary acceptor vector for plant cell transformation, with a tetracycline-resistance gene.

 
To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

 KpnI (45) AvrII (20,349) MauBI (19,473) virD1 SnaBI (5139) FseI (8462) BsiWI (11,188) KpnI (14,854) BstZ17I (15,048) PciI (15,275) SbfI (16,851) pSB1 36,909 bp
KpnI  (45)
2 sites
G G T A C C C C A T G G
AvrII  (20,349)
1 site
C C T A G G G G A T C C
MauBI  (19,473)
1 site
C G C G C G C G G C G C G C G C
SnaBI  (5139)
1 site
T A C G T A A T G C A T
FseI  (8462)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
BsiWI  (11,188)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
KpnI  (14,854)
2 sites
G G T A C C C C A T G G
BstZ17I  (15,048)
1 site
G T A T A C C A T A T G
PciI  (15,275)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
SbfI  (16,851)
1 site
C C T G C A G G G G A C G T C C
incC,korABFG operon
31,395 .. 34,828  =  3434 bp
incC,korABFG operon
31,395 .. 34,828  =  3434 bp
virB4
9057 .. 11,426  =  2370 bp
789 amino acids  =  87.4 kDa
Product: VirB4
integral inner membrane ATPase
virB4
9057 .. 11,426  =  2370 bp
789 amino acids  =  87.4 kDa
Product: VirB4
integral inner membrane ATPase
kfrBC operon
28,439 .. 30,352  =  1914 bp
kfrBC operon
28,439 .. 30,352  =  1914 bp
TcR
19,088 .. 20,287  =  1200 bp
399 amino acids  =  42.2 kDa
Product: tetracycline efflux protein
confers resistance to tetracycline
TcR
19,088 .. 20,287  =  1200 bp
399 amino acids  =  42.2 kDa
Product: tetracycline efflux protein
confers resistance to tetracycline
trfA
21,560 .. 22,708  =  1149 bp
382 amino acids  =  43.8 kDa
Product: trans-acting replication protein that binds
to and activates oriV
trfA
21,560 .. 22,708  =  1149 bp
382 amino acids  =  43.8 kDa
Product: trans-acting replication protein that binds
to and activates oriV
virB10
4487 .. 5620  =  1134 bp
377 amino acids  =  40.5 kDa
Product: VirB10
T-DNA transfer
virB10
4487 .. 5620  =  1134 bp
377 amino acids  =  40.5 kDa
Product: VirB10
T-DNA transfer
virB11
3416 .. 4447  =  1032 bp
343 amino acids  =  38.0 kDa
Product: VirB11
T-DNA transfer
virB11
3416 .. 4447  =  1032 bp
343 amino acids  =  38.0 kDa
Product: VirB11
T-DNA transfer
virB6
7396 .. 8283  =  888 bp
295 amino acids  =  31.8 kDa
Product: VirB6
T-DNA transfer
virB6
7396 .. 8283  =  888 bp
295 amino acids  =  31.8 kDa
Product: VirB6
T-DNA transfer
virG
2481 .. 3284  =  804 bp
267 amino acids  =  30.0 kDa
Product: VirG
response regulator of virA/virG two component
transduction system
virG
2481 .. 3284  =  804 bp
267 amino acids  =  30.0 kDa
Product: VirG
response regulator of virA/virG two component
transduction system
virB1
12,132 .. 12,851  =  720 bp
239 amino acids  =  25.9 kDa
Product: VirB1
T-DNA transfer
virB1
12,132 .. 12,851  =  720 bp
239 amino acids  =  25.9 kDa
Product: VirB1
T-DNA transfer
virB8
6495 .. 7208  =  714 bp
237 amino acids  =  26.2 kDa
Product: VirB8
T-DNA transfer
virB8
6495 .. 7208  =  714 bp
237 amino acids  =  26.2 kDa
Product: VirB8
T-DNA transfer
oriV
35,754 .. 36,465  =  712 bp
incP origin of replication
oriV
35,754 .. 36,465  =  712 bp
incP origin of replication
virC1 (inactivated)
1065 .. 1759  =  695 bp
Product: VirC1, inactivated by a frameshift mutation
that eliminated the final C in codon Thr-56
virC1 (inactivated)
1065 .. 1759  =  695 bp
Product: VirC1, inactivated by a frameshift mutation
that eliminated the final C in codon Thr-56
virB5
8380 .. 9042  =  663 bp
220 amino acids  =  23.5 kDa
Product: VirB5
T-DNA transfer
virB5
8380 .. 9042  =  663 bp
220 amino acids  =  23.5 kDa
Product: VirB5
T-DNA transfer
TetR
18,332 .. 18,982  =  651 bp
216 amino acids  =  23.3 kDa
Product: tetracycline resistance regulatory protein
TetR
18,332 .. 18,982  =  651 bp
216 amino acids  =  23.3 kDa
Product: tetracycline resistance regulatory protein
virC2
1762 .. 2370  =  609 bp
202 amino acids  =  22.4 kDa
Product: VirC2
virC2
1762 .. 2370  =  609 bp
202 amino acids  =  22.4 kDa
Product: VirC2
ori
15,336 .. 15,924  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
15,336 .. 15,924  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
virD1
353 .. 796  =  444 bp
147 amino acids  =  16.3 kDa
Product: VirD1
cleavage accessory protein
virD1
353 .. 796  =  444 bp
147 amino acids  =  16.3 kDa
Product: VirD1
cleavage accessory protein
cos
16,432 .. 16,830  =  399 bp
lambda cos site; allows packaging into phage
lambda particles
cos
16,432 .. 16,830  =  399 bp
lambda cos site; allows packaging into phage
lambda particles
traJ
26,118 .. 26,489  =  372 bp
123 amino acids  =  13.5 kDa
Product: oriT-recognizing protein
traJ
26,118 .. 26,489  =  372 bp
123 amino acids  =  13.5 kDa
Product: oriT-recognizing protein
virB2
11,752 .. 12,117  =  366 bp
121 amino acids  =  12.3 kDa
Product: VirB2
pilin protein precursor
virB2
11,752 .. 12,117  =  366 bp
121 amino acids  =  12.3 kDa
Product: VirB2
pilin protein precursor
bom
15,010 .. 15,150  =  141 bp
basis of mobility region from pBR322
bom
15,010 .. 15,150  =  141 bp
basis of mobility region from pBR322
oriT
26,522 .. 26,631  =  110 bp
incP origin of transfer
oriT
26,522 .. 26,631  =  110 bp
incP origin of transfer
kfrA operon
30,253 .. 31,335  =  1083 bp
kfrA operon
30,253 .. 31,335  =  1083 bp
virB9
5617 .. 6498  =  882 bp
293 amino acids  =  32.1 kDa
Product: VirB9
T-DNA transfer
virB9
5617 .. 6498  =  882 bp
293 amino acids  =  32.1 kDa
Product: VirB9
T-DNA transfer
virB3
11,426 .. 11,752  =  327 bp
108 amino acids  =  11.8 kDa
Product: VirB3
T-DNA transfer
virB3
11,426 .. 11,752  =  327 bp
108 amino acids  =  11.8 kDa
Product: VirB3
T-DNA transfer
virB7
7195 .. 7362  =  168 bp
55 amino acids  =  5.8 kDa
Product: VirB7
T-DNA transfer
virB7
7195 .. 7362  =  168 bp
55 amino acids  =  5.8 kDa
Product: VirB7
T-DNA transfer
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter