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Plasmid Files

pDrive

Bacterial vector for UA cloning and expression of a PCR product.

To see this sequence with restriction sites, features, and translations, please download
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pDrive.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Qiagen
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BspQI - SapI (3842) PciI (3725) PspFI (3425) BseYI (3421) AlwNI (3316) PflMI (2875) Bpu10I (2629) BsmBI (2628) AsiSI (2612) EcoNI (2524) SmaI (2486) TspMI - XmaI (2484) BspDI - ClaI (2303) NruI (2269) AhdI (1962) Acc65I (266) KpnI (270) SphI (278) PstI (283) MluI (285) SnaBI (293) BamHI (299) EcoRI (306) EcoRV (316) EcoRI (321) SalI (327) AccI (328) HincII (329) HindIII (333) PaeR7I - XhoI (339) AvrII - StyI (345) NheI (350) BmtI (354) XbaI (356) AleI - PmlI (367) BstXI (369) EcoO109I - PspOMI (374) ApaI (378) Eco53kI (382) SacI (384) EagI - NotI (387) AanI - PsiI (684) DraIII (812) BtgZI (813) NgoMIV (913) NaeI (915) BsaHI (1422) TatI (1479) ScaI (1481) NmeAIII (1815) BpmI (1893) BsaI (1896) pDrive 3851 bp
BspQI  (3842)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (3842)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
PciI  (3725)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
PspFI  (3425)
1 site
C C C A G C G G G T C G
BseYI  (3421)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
AlwNI  (3316)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PflMI  (2875)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
Bpu10I  (2629)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BsmBI  (2628)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
AsiSI  (2612)
1 site
G C G A T C G C C G C T A G C G
EcoNI  (2524)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
SmaI  (2486)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (2484)
1 site
C C C G G G G G G C C C
XmaI  (2484)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
BspDI  (2303)
1 site
A T C G A T T A G C T A
ClaI  (2303)
1 site
A T C G A T T A G C T A
NruI  (2269)
1 site
T C G C G A A G C G C T
AhdI  (1962)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
Acc65I  (266)
1 site
G G T A C C C C A T G G
KpnI  (270)
1 site
G G T A C C C C A T G G
SphI  (278)
1 site
G C A T G C C G T A C G
PstI  (283)
1 site
C T G C A G G A C G T C
MluI  (285)
1 site
A C G C G T T G C G C A
SnaBI  (293)
1 site
T A C G T A A T G C A T
BamHI  (299)
1 site
G G A T C C