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Plasmid Files

pQE-60

Bacterial vector for expressing C-terminally 6xHis-tagged proteins, with an NcoI site at the start codon.

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pQE-60.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Qiagen
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BbsI (3417) EcoO109I (3415) AatII (3361) ZraI (3359) XmnI (3038) PvuI (2809) FspI (2661) AseI (2611) NmeAIII (2587) BglI (2559) BsrFI (2519) BsaI (2500) AhdI (2439) AlwNI (1962) PspFI (1854) BseYI (1850) AvaI - BsoBI - PaeR7I - XhoI (1) BmeT110I (2) PsiI (49) MfeI (59) EcoRI (88) BtgI - NcoI - StyI (113) BseRI (115) BamHI (121) BsaBI * (126) BglII (127) HindIII (152) BlpI (164) NheI (272) BmtI (276) Bpu10I (297) PvuII (424) BspEI (520) PasI - PflMI * (756) MscI (791) NsiI (831) XbaI (1133) PfoI (1190) PflFI - Tth111I (1293) BsaAI (1300) AccI (1318) BstZ17I (1319) NdeI (1369) BstAPI (1370) BspQI - SapI (1430) AflIII - PciI (1546) pQE-60 3431 bp
BbsI  (3417)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
EcoO109I  (3415)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
AatII  (3361)
1 site
G A C G T C C T G C A G
ZraI  (3359)
1 site
G A C G T C C T G C A G
XmnI  (3038)
1 site
G A A N N N N T T C C T T N N N N A A G
PvuI  (2809)
1 site
C G A T C G G C T A G C
FspI  (2661)
1 site
T G C G C A A C G C G T
AseI  (2611)
1 site
A T T A A T T A A T T A
NmeAIII  (2587)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BglI  (2559)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BsrFI  (2519)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
BsaI  (2500)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (2439)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (1962)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PspFI  (1854)
1 site
C C C A G C G G G T C G
BseYI  (1850)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
AvaI  (1)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (1)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
PaeR7I  (1)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1)
1 site
C T C G A G G A G C T C
BmeT110I  (2)
1 site
C Y C G R G G R G C Y C
PsiI  (49)
1 site
T T A T A A A A T A T T
MfeI  (59)
1 site
C A A T T G G T T A A C
EcoRI  (88)
1 site
G A A T T C C T T A A G
BtgI  (113)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (113)
1 site
C C A T G G G G T A C C