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Plasmid Files

pQE-82L

Bacterial lacIq vector for expressing N-terminally 6xHis-tagged proteins. For other reading frames, use pQE‑80L or pQE‑81L.

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pQE-82L.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Qiagen
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AatII (4682) ZraI (4680) XmnI (4359) PvuI (4130) BglI (3880) BsaI (3821) AhdI (3760) AlwNI (3283) PciI (2867) BspQI - SapI (2751) NdeI (2690) BstZ17I (2640) PflFI - Tth111I (2614) PfoI (2511) FspAI (2447) PaeR7I - XhoI (1) PsiI (49) MfeI (59) EcoRI (88) BseRI (115) BamHI (146) SphI (156) Eco53kI (160) SacI (162) Acc65I (164) KpnI (168) TspMI - XmaI (169) SmaI (171) SalI (174) PstI (184) BfuAI - BspMI (187) HindIII (188) BlpI (200) NheI (308) BmtI (312) Bpu10I (333) BspEI (556) PasI (792) KasI (1338) NarI * (1339) SfoI (1340) PluTI (1342) HpaI (1475) EcoRV (1531) BssHII (1566) PspOMI (1770) ApaI (1774) BstEII (1795) BclI * (1963) MluI (1977) PpuMI * (2430) pQE-82L 4752 bp
AatII  (4682)
1 site
G A C G T C C T G C A G
ZraI  (4680)
1 site
G A C G T C C T G C A G
XmnI  (4359)
1 site
G A A N N N N T T C C T T N N N N A A G
PvuI  (4130)
1 site
C G A T C G G C T A G C
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BglI  (3880)
1 site
G C C N N N N N G G C C G G N N N N N C C G