pQE-TriSystem His-Strep1

Vector for expression in bacterial, insect, and vertebrate cells of proteins with C-terminal Strep-Tag II and 8xHis tags.

Sequence Author: Qiagen

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Qiagen Vectors | More Plasmid Sets
No matches
AanI (5163) AanI (5074) AanI (3124) AanI (1111) NcoI (1265) EcoRV (1274) TspMI - XmaI (1277) SmaI (1279) Eco53kI (1284) SacI (1286) BamHI (1290) EcoRI (1296) BglII (1304) BfuAI - BspMI (1311) AscI (1312) PstI - SbfI (1322) Acc65I (1330) KpnI (1334) HindIII (1342) EagI - NotI (1349) PvuII (1359) BstZ17I (1364) PmlI (1369) PaeR7I - PspXI - XhoI (1405) AanI (1981) pQE-TriSystem His-Strep1 5760 bp
AanI  (5163)
5 sites
T T A T A A A A T A T T
AanI  (5074)
5 sites
T T A T A A A A T A T T
AanI  (3124)
5 sites
T T A T A A A A T A T T
AanI  (1111)
5 sites
T T A T A A A A T A T T
NcoI  (1265)
1 site
C C A T G G G G T A C C
EcoRV  (1274)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
TspMI  (1277)
1 site
C C C G G G G G G C C C
XmaI  (1277)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (1279)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
Eco53kI  (1284)
1 site
G A G C T C C T C G A G
SacI  (1286)
1 site
G A G C T C C T C G A G
BamHI  (1290)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
EcoRI  (1296)
1 site
G A A T T C C T T A A G
BglII  (1304)
1 site
A G A T C T T C T A G A
BfuAI  (1311)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (1311)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
AscI  (1312)
1 site
G G C G C G C C C C G C G C G G
PstI  (1322)
1 site
C T G C A G G A C G T C
SbfI  (1322)
1 site
C C T G C A G G G G A C G T C C
Acc65I  (1330)
1 site
G G T A C C C C A T G G
KpnI  (1334)
1 site
G G T A C C C C A T G G
HindIII  (1342)
1 site
A A G C T T T T C G A A
EagI  (1349)
1 site
C G G C C G G C C G G C
NotI  (1349)
1 site
G C G G C C G C C G C C G G C G
PvuII  (1359)
1 site
C A G C T G G T C G A C
BstZ17I  (1364)
1 site
G T A T A C C A T A T G
PmlI  (1369)
1 site
C A C G T G G T G C A C
PaeR7I  (1405)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (1405)
1 site
V C T C G A G B B G A G C T C V
XhoI  (1405)
1 site
C T C G A G G A G C T C
AanI  (1981)
5 sites
T T A T A A A A T A T T
baculovirus recombination region (ORF1629)
1792 .. 3250  =  1459 bp
contains part of ORF1629
baculovirus recombination region (ORF1629)
1792 .. 3250  =  1459 bp
contains part of ORF1629
Exon 1
651 .. 742  =  92 bp
Exon 1
651 .. 742  =  92 bp
Intron
743 .. 1004  =  262 bp
Intron
743 .. 1004  =  262 bp
Exon 2
1005 .. 1613  =  609 bp
Exon 2
1005 .. 1613  =  609 bp
AmpR
4009 .. 4869  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   4009 .. 4800  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4009 .. 4869  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   4801 .. 4869  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4009 .. 4869  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
baculovirus recombination region (lef2/ORF603)
4935 .. 2  =  828 bp
contains ORF603 and part of lef2
baculovirus recombination region (lef2/ORF603)
4935 .. 2  =  828 bp
contains ORF603 and part of lef2
ori
3409 .. 3996  =  588 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3409 .. 3996  =  588 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CMV enhancer
62 .. 367  =  306 bp
human cytomegalovirus (CMV) immediate early enhancer
CMV enhancer
62 .. 367  =  306 bp
human cytomegalovirus (CMV) immediate early enhancer
chicken β-actin promoter
373 .. 650  =  278 bp
chicken β-actin promoter
373 .. 650  =  278 bp
T7 terminator
1732 .. 1779  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
T7 terminator
1732 .. 1779  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
ORF1629
1909 .. 3250  =  1342 bp
446 amino acids  =  49.2 kDa
Product: baculovirus capsid-associated protein
required for viral replication
ORF1629
1909 .. 3250  =  1342 bp
446 amino acids  =  49.2 kDa
Product: baculovirus capsid-associated protein
required for viral replication
ORF603
5097 .. 5702  =  606 bp
201 amino acids  =  23.6 kDa
Product: baculovirus ORF603 protein
ORF603
5097 .. 5702  =  606 bp
201 amino acids  =  23.6 kDa
Product: baculovirus ORF603 protein
ATG
1267 .. 1269  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
1267 .. 1269  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
Strep-Tag II
1381 .. 1404  =  24 bp
8 amino acids  =  1.1 kDa
Product: peptide that binds Strep-Tactin, an engineered form of streptavidin
Strep-Tag II
1381 .. 1404  =  24 bp
8 amino acids  =  1.1 kDa
Product: peptide that binds Strep-Tactin, an engineered form of streptavidin
8xHis
1411 .. 1434  =  24 bp
8 amino acids  =  1.1 kDa
Product: 8xHis affinity tag
8xHis
1411 .. 1434  =  24 bp
8 amino acids  =  1.1 kDa
Product: 8xHis affinity tag
lef2
4935 .. 5059  =  125 bp
40 amino acids  =  4.5 kDa
Product: baculovirus late expression factor 2
lef2
4935 .. 5059  =  125 bp
40 amino acids  =  4.5 kDa
Product: baculovirus late expression factor 2
rabbit globin terminator
1611 .. 1722  =  112 bp
rabbit globin terminator
1611 .. 1722  =  112 bp
p10 promoter
1143 .. 1252  =  110 bp
baculovirus promoter for expression in insect cells
p10 promoter
1143 .. 1252  =  110 bp
baculovirus promoter for expression in insect cells
T5 promoter
1073 .. 1116  =  44 bp
4 segments
   Segment 1:  
   1073 .. 1086  =  14 bp
bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator
T5 promoter
1073 .. 1116  =  44 bp
4 segments
   Segment 2:  -35  
   1087 .. 1092  =  6 bp
bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator
T5 promoter
1073 .. 1116  =  44 bp
4 segments
   Segment 3:  
   1093 .. 1109  =  17 bp
bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator
T5 promoter
1073 .. 1116  =  44 bp
4 segments
   Segment 4:  -10  
   1110 .. 1116  =  7 bp
bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator
T5 promoter
1073 .. 1116  =  44 bp
4 segments
bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator
stop codons
1435 .. 1445  =  11 bp
stop codons in all three reading frames
stop codons
1435 .. 1445  =  11 bp
stop codons in all three reading frames
RBS
1255 .. 1260  =  6 bp
ribosome binding site
RBS
1255 .. 1260  =  6 bp
ribosome binding site
poly(A) signal
1589 .. 1594  =  6 bp
poly(A) signal
1589 .. 1594  =  6 bp
MCS
1265 .. 1372  =  108 bp
multiple cloning site
MCS
1265 .. 1372  =  108 bp
multiple cloning site
lac operator
1092 .. 1108  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
1092 .. 1108  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
ORF:  239 .. 628  =  390 bp
ORF:  129 amino acids  =  13.9 kDa
ORF:  4139 .. 4405  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  471 .. 725  =  255 bp
ORF:  84 amino acids  =  8.6 kDa
ORF:  1234 .. 1575  =  342 bp
ORF:  113 amino acids  =  12.8 kDa
ORF:  1909 .. 3162  =  1254 bp
ORF:  417 amino acids  =  46.0 kDa
ORF:  4009 .. 4869  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  456 .. 983  =  528 bp
ORF:  175 amino acids  =  18.5 kDa
ORF:  5097 .. 5702  =  606 bp
ORF:  201 amino acids  =  23.6 kDa
ORF:  5141 .. 5371  =  231 bp
ORF:  76 amino acids  =  9.2 kDa
Click here to try SnapGene

Download pQE-TriSystem His-Strep1.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.