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Plasmid Files

pLenti7.3/V5-TOPO® (linearized)

Linearized lentiviral expression vector with 3'-T overhangs and bound topoisomerase, for TOPO® TA cloning of PCR products and co-expression with GFP.

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pLenti7.3 V5-TOPO (linearized).dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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End (7935) Start (1) pLenti7.3/V5-TOPO® 7934 bp
End  (7935)
0 sites
Start  (1)
0 sites
AmpR
3288 .. 4148  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   3288 .. 3356  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3288 .. 4148  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   3357 .. 4148  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3288 .. 4148  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
EmGFP
1103 .. 1822  =  720 bp
239 amino acids  =  26.9 kDa
   Segment 1:  
   1103 .. 1105  =  3 bp
   1 amino acid  =  149.2 Da
Product: Emerald GFP
mammalian codon-optimized
EmGFP
1103 .. 1822  =  720 bp
239 amino acids  =  26.9 kDa
   Segment 2:  1a  
   1106 .. 1108  =  3 bp
   1 amino acid  =  117.2 Da
Product: Emerald GFP
mammalian codon-optimized
EmGFP
1103 .. 1822  =  720 bp
239 amino acids  =  26.9 kDa
   Segment 3:  
   1109 .. 1822  =  714 bp
   237 amino acids  =  26.7 kDa
Product: Emerald GFP
mammalian codon-optimized
EmGFP
1103 .. 1822  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
Product: Emerald GFP
mammalian codon-optimized
WPRE
133 .. 721  =  589 bp
woodchuck posttranscriptional regulatory element
WPRE
133 .. 721  =  589 bp
woodchuck posttranscriptional regulatory element
ori
4319 .. 4907  =  589 bp
high-copy-number colE1/pMB1/pBR322/pUC origin of replication
ori
4319 .. 4907  =  589 bp
high-copy-number colE1/pMB1/pBR322/pUC origin of replication
f1 ori
2702 .. 3157  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
2702 .. 3157  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
SV40 promoter
736 .. 1052  =  317 bp
SV40 enhancer and early promoter
SV40 promoter
736 .. 1052  =  317 bp
SV40 enhancer and early promoter
CMV enhancer
7317 .. 7620  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
7317 .. 7620  =  304 bp
human cytomegalovirus immediate early enhancer
3'LTR (ΔU3)
1894 .. 2127  =  234 bp
self-inactivating 3' long terminal repeat (LTR) from HIV-1
3'LTR (ΔU3)
1894 .. 2127  =  234 bp
self-inactivating 3' long terminal repeat (LTR) from HIV-1
HIV-1 RRE
6448 .. 6681  =  234 bp
The Rev response element (RRE) of human immunodeficiency virus type 1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
HIV-1 RRE
6448 .. 6681  =  234 bp
The Rev response element (RRE) of human immunodeficiency virus type 1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
RSV promoter
5374 .. 5597  =  224 bp
Rous sarcoma virus promoter
RSV promoter
5374 .. 5597  =  224 bp
Rous sarcoma virus promoter
CMV promoter
7621 .. 7824  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
7621 .. 7824  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
5' LTR
5603 .. 5783  =  181 bp
truncated 5' long terminal repeat (LTR) from HIV-1
5' LTR
5603 .. 5783  =  181 bp
truncated 5' long terminal repeat (LTR) from HIV-1
HIV-1 ψ
5830 .. 5955  =  126 bp
packaging signal of human immunodeficiency virus type 1
HIV-1 ψ
5830 .. 5955  =  126 bp
packaging signal of human immunodeficiency virus type 1
SV40 poly(A) signal
2199 .. 2320  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
2199 .. 2320  =  122 bp
SV40 polyadenylation signal
HIV-1 cPPT
7177 .. 7294  =  118 bp
central polypurine tract from pol gene of human immunodeficiency virus type 1
HIV-1 cPPT
7177 .. 7294  =  118 bp
central polypurine tract from pol gene of human immunodeficiency virus type 1
AmpR promoter
3183 .. 3287  =  105 bp
AmpR promoter
3183 .. 3287  =  105 bp
V5 tag
68 .. 109  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
V5 tag
68 .. 109  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
SV40 ori
903 .. 1038  =  136 bp
SV40 origin of replication
SV40 ori
903 .. 1038  =  136 bp
SV40 origin of replication
ORF:  124 .. 882  =  759 bp
ORF:  252 amino acids  =  27.2 kDa
ORF:  2221 .. 2457  =  237 bp
ORF:  78 amino acids  =  8.6 kDa
ORF:  5305 .. 5550  =  246 bp
ORF:  81 amino acids  =  8.9 kDa
ORF:  236 .. 532  =  297 bp
ORF:  98 amino acids  =  10.2 kDa
ORF:  728 .. 1000  =  273 bp
ORF:  90 amino acids  =  10.3 kDa
ORF:  1103 .. 1822  =  720 bp
ORF:  239 amino acids  =  26.9 kDa
ORF:  6326 .. 7336  =  1011 bp
ORF:  336 amino acids  =  38.5 kDa
ORF:  543 .. 797  =  255 bp
ORF:  84 amino acids  =  8.9 kDa
ORF:  3288 .. 4148  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  6241 .. 6621  =  381 bp
ORF:  126 amino acids  =  13.3 kDa
ORF:  5232 .. 5531  =  300 bp
ORF:  99 amino acids  =  10.9 kDa
ORF:  3752 .. 4018  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  7703 .. 7933  =  231 bp
ORF:  76 amino acids  =  8.2 kDa  (no start codon)
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