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Plasmid Files

pSpark® TA

Linearized vector with 3'-T overhangs and a classical multiple cloning site for TA cloning of PCR products.

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pSpark TA.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Canvax Biotech
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PspOMI (2960) NaeI (2642) NgoMIV (2640) BtgZI (2540) BsaAI - DraIII (2539) PsiI (2411) XmnI (1944) ScaI (1825) TatI (1823) NmeAIII (1493) BpmI (1415) ApaI (2964) ZraI (2968) AatII (2970) SphI (2976) BstZI (2981) NcoI - StyI (2987) SfiI (2989) SacII (2996) End (3002) Start (1) SpeI (5) BfuAI - BspMI - BstZI - NotI (12) PstI - SbfI (23) SalI (25) AccI (26) HincII (27) NdeI (32) Eco53kI (42) SacI (44) MluI (49) BstXI (53) NsiI (62) BspQI - SapI (336) PciI (452) BseYI (756) PspFI (760) AlwNI (868) AhdI (1345) BsaI (1406) pSpark® TA 3001 bp
PspOMI  (2960)
1 site
G G G C C C C C C G G G
NaeI  (2642)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (2640)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
BtgZI  (2540)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BsaAI  (2539)
1 site
Y A C G T R R T G C A Y
DraIII  (2539)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PsiI  (2411)
1 site
T T A T A A A A T A T T
XmnI  (1944)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (1825)
1 site
A G T A C T T C A T G A
TatI  (1823)
1 site
W G T A C W W C A T G W
NmeAIII  (1493)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BpmI  (1415)
1 site
C T G G A G ( N ) 14 N N G A C C T