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Plasmid Files

pcDNA™3.1/CT-GFP-TOPO® (linearized)

Linearized vector with 3'-T overhangs and bound topoisomerase, for TOPO® TA cloning to add a C-terminal GFP tag.

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pcDNA3.1 CT-GFP-TOPO (linearized).dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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SgrDI (5202) SspI (5085) Acc113I - BmcAI - ScaI - ZrmI (4761) BpvUI - Ple19I - PvuI (4651) AhdI - AspEI - BmeRI - DriI - Eam1105I (4281) BspLU11I - PciI - PscI (3388) BsaMI - BsmI - Mva1269I - PctI (2957) PfoI (2812) CpoI - CspI - RsrII - Rsr2I (2551) BsePI - BssHII - PauI - PteI (2432) AspI - PflFI - PsyI - Tth111I (2153) BbeI - PluTI (2038) DinI - EgeI - EheI - SfoI (2036) Mly113I - NarI (2035) KasI - SspDI (2034) BglII (5216) Bpu10I (5384) BstSNI - Eco105I - SnaBI (5794) Acc65I - Asp718I (6112) KpnI (6116) End (6157) Start (1) Eco32I - EcoRV (18) CciNI - NotI (33) XbaI (45) AsuNHI - NheI (54) BmtI - BspOI (58) AccIII * - Aor13HI - BseAI * - Bsp13I - BspEI * - Kpn2I - MroI (275) Bsp1407I - BsrGI - BstAUI - SspBI (329) AsuII - Bpu14I - Bsp119I - BspT104I - BstBI - Csp45I - NspV - SfuI (675) BbsI - BpiI - BpuAI - BstV2I (987) AdeI - DraIII (1301) CsiI - MabI - SexAI * (1592) BseRI (1821) AatI - Eco147I - PceI - SseBI - StuI (1824) AspA2I - AvrII - BlnI - XmaJI (1825) Cfr9I - TspMI - XmaI - XmaCI (1846) SmaI (1848) BsaBI * - Bse8I - BseJI * - MamI * (1894)