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Plasmid Files

pAdenoX-ZsGreen1 Linear

In-Fusion®-ready linearized adenoviral vector for constitutive expression of a gene together with the fluorescent protein ZsGreen1.

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pAdenoX-ZsGreen1 Linear Sequence and MappAdenoX-ZsGreen1 Linear.dna
Map and Sequence File   
Sequence Author:  Clontech
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 End (35,866) In-Fusion Cloning site SgrDI (35,844) CMV promoter Adeno-X fwd screening primer (34,977 .. 35,001) ITR M13 rev AmpR promoter BstBI (31,906) SV40 poly(A) signal CMV promoter I-PpoI (25,302) SrfI (24,953) SpeI (24,505) MreI (23,818) AsiSI (18,871) Start (0) In-Fusion Cloning site stop codons Adeno-X rev screening primer (936 .. 955) BstZ17I (3197) PmeI (10,687) pAdenoX-ZsGreen1 Linear 35,866 bp
End  (35,866)
0 sites
SgrDI  (35,844)
1 site
C G T C G A C G G C A G C T G C
BstBI  (31,906)
1 site
T T C G A A A A G C T T
I-PpoI  (25,302)
1 site
C T C T C T T A A G G T A G C G A G A G A A T T C C A T C G

I-PpoI is a homing endonuclease that can recognize a variety of
similar recognition sequences.
SrfI  (24,953)
1 site
G C C C G G G C C G G G C C C G
SpeI  (24,505)
1 site
A C T A G T T G A T C A
MreI  (23,818)
1 site
C G C C G G C G G C G G C C G C
AsiSI  (18,871)
1 site
G C G A T C G C C G C T A G C G
Start  (0)
0 sites
BstZ17I  (3197)
1 site
G T A T A C C A T A T G
PmeI  (10,687)
1 site
G T T T A A A C C A A A T T T G
Adeno-X fwd screening primer
25-mer  /  52% GC
1 binding site
34,977 .. 35,001  =  25 annealed bases
Tm  =  63°C
Adeno-X rev screening primer
20-mer  /  50% GC
1 binding site
936 .. 955  =  20 annealed bases
Tm  =  57°C
adenovirus DNA
956 .. 25,286  =  24,331 bp
adenovirus DNA
956 .. 25,286  =  24,331 bp
adenovirus DNA
26,946 .. 31,781  =  4836 bp
adenovirus DNA
26,946 .. 31,781  =  4836 bp
AmpR
32,785 .. 33,645  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   32,785 .. 32,853  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
32,785 .. 33,645  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   32,854 .. 33,645  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
32,785 .. 33,645  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
ZsGreen1
25,966 .. 26,661  =  696 bp
231 amino acids  =  26.1 kDa
Product: Zoanthus green fluorescent protein
mammalian codon-optimized
ZsGreen1
25,966 .. 26,661  =  696 bp
231 amino acids  =  26.1 kDa
Product: Zoanthus green fluorescent protein
mammalian codon-optimized
ori
33,816 .. 34,404  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
33,816 .. 34,404  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
f1 ori
32,146 .. 32,601  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
32,146 .. 32,601  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
CMV enhancer
25,413 .. 25,716  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
25,413 .. 25,716  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
35,314 .. 35,617  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
35,314 .. 35,617  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
25,717 .. 25,920  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
25,717 .. 25,920  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
35,618 .. 35,821  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
35,618 .. 35,821  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
Ad5 Ψ
35,064 .. 35,214  =  151 bp
packaging signal for adenovirus serotype 5
Ad5 Ψ
35,064 .. 35,214  =  151 bp
packaging signal for adenovirus serotype 5
AmpR promoter
32,680 .. 32,784  =  105 bp
AmpR promoter
32,680 .. 32,784  =  105 bp
ITR
31,782 .. 31,884  =  103 bp
inverted terminal repeat of human adenovirus
serotype 5
ITR
31,782 .. 31,884  =  103 bp
inverted terminal repeat of human adenovirus
serotype 5
ITR
34,874 .. 34,976  =  103 bp
inverted terminal repeat of human adenovirus
serotype 5
ITR
34,874 .. 34,976  =  103 bp
inverted terminal repeat of human adenovirus
serotype 5
SV40 poly(A) signal
822 .. 903  =  82 bp
SV40 polyadenylation signal
SV40 poly(A) signal
822 .. 903  =  82 bp
SV40 polyadenylation signal
SV40 poly(A) signal
26,783 .. 26,864  =  82 bp
SV40 polyadenylation signal
SV40 poly(A) signal
26,783 .. 26,864  =  82 bp
SV40 polyadenylation signal
small t intron
182 .. 247  =  66 bp
simian virus 40 (SV40) small t antigen intron
small t intron
182 .. 247  =  66 bp
simian virus 40 (SV40) small t antigen intron
M13 fwd
31,989 .. 32,005  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 fwd
31,989 .. 32,005  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 rev
34,790 .. 34,806  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 rev
34,790 .. 34,806  =  17 bp
common sequencing primer, one of multiple similar
variants
In-Fusion Cloning site
1 .. 15  =  15 bp
sequence to be added to 5' end of reverse primer
In-Fusion Cloning site
1 .. 15  =  15 bp
sequence to be added to 5' end of reverse primer
In-Fusion Cloning site
35,852 .. 35,866  =  15 bp
sequence to be added to 5' end of forward primer
In-Fusion Cloning site
35,852 .. 35,866  =  15 bp
sequence to be added to 5' end of forward primer
stop codons
35 .. 45  =  11 bp
stop codons in all three reading frames
stop codons
35 .. 45  =  11 bp
stop codons in all three reading frames
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