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pLVX-Het-1

Lentiviral vector encoding an FRB heterodimerizer domain, for creating soluble fusion proteins that can be dimerized with a drug.

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pLVX-Het-1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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SgrDI (8069) SspI (7952) PvuI (7518) FspI (7370) AhdI (7148) DrdI (6366) BmtI (5874) NheI (5870) Bsu36I (5080) PstI (4964) MluI (4346) SexAI * (4313) XcmI (8294) NruI * (833) FseI (1150) MfeI (1189) BbvCI - Bpu10I (1424) AleI (1577) KflI (1934) BspDI - ClaI (2180) NdeI (2374) EcoRI (2804) PaeR7I - XhoI (2810) SpeI (2816) XbaI (2828) SphI (2939) NotI (3115) BamHI (3122) AvrII (3291) AarI - BfuAI - BspMI (3479) BmgBI (3683) PflFI - Tth111I (3788) TspMI - XmaI (3795) SmaI (3797) BsiWI (3802) RsrII (3862) BstEII (3880) MscI * (4121) pLVX-Het-1 8437 bp
SgrDI  (8069)
1 site
C G T C G A C G G C A G C T G C
SspI  (7952)
1 site
A A T A T T T T A T A A
PvuI  (7518)
1 site
C G A T C G G C T A G C
FspI  (7370)
1 site
T G C G C A A C G C G T
AhdI  (7148)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
DrdI  (6366)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
BmtI  (5874)
1 site
G C T A G C C G A T C G
NheI  (5870)
1 site
G C T A G C C G A T C G
Bsu36I  (5080)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
PstI  (4964)
1 site
C T G C A G G A C G T C
MluI  (4346)
1 site
A C G C G T T G C G C A
SexAI  (4313)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
XcmI  (8294)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
NruI  (833)
1 site
T C G C G A A G C G C T
* Blocked by Dam methylation.
FseI  (1150)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
MfeI  (1189)
1 site
C A A T T G G T T A A C
BbvCI  (1424)
1 site
C C T C A G C G G A G T C G
Bpu10I  (1424)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
AleI  (1577)
1 site
C A C N N N N G T G G T G N N N N C A C
KflI  (1934)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
BspDI  (2180)
1 site
A T C G A T T A G C T A
ClaI  (2180)
1 site
A T C G A T T A G C T A
NdeI  (2374)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
EcoRI  (2804)
1 site
G A A T T C C T T A A G
PaeR7I  (2810)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (2810)
1 site
C T C G A G G A G C T C
SpeI  (2816)
1 site
A C T A G T T G A T C A
XbaI  (2828)
1 site
T C T A G A A G A T C T
SphI  (2939)
1 site
G C A T G C C G T A C G
NotI  (3115)
1 site
G C G G C C G C C G C C G G C G
BamHI  (3122)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
AvrII  (3291)
1 site
C C T A G G G G A T C C
AarI  (3479)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.
BfuAI  (3479)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (3479)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BmgBI  (3683)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
PflFI  (3788)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3788)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
TspMI  (3795)
1 site
C C C G G G G G G C C C
XmaI  (3795)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
SmaI  (3797)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
BsiWI  (3802)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
RsrII  (3862)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BstEII  (3880)
1 site
G G T N A