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pLenti-C-Myc-DDK-IRES-Neo

Third-generation lentiviral PrecisionShuttle™ vector with a neomycin resistance marker, for fusing an ORF to C-terminal c-Myc and FLAG® tags.

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pLenti-C-Myc-DDK-IRES-Neo.dna
Map and Sequence File:    Download    Open   
Sequence Author:  OriGene
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BsmBI (7219) PasI (7212) ScaI (7030) Bsu36I (5700) SacII (5481) BsrGI (4860) SphI (4598) PflFI - Tth111I (4311) FspI (4295) BclI * (4058) BmgBI (4033) SgrDI (1) FseI (1530) EcoNI (1550) BbvCI (1804) AleI (1957) XbaI (2613) EcoRI (3279) BamHI (3292) AsiSI - SgfI (3324) MreI - SgrAI (3326) AscI (3330) NheI (3355) BmtI (3359) RsrII (3361) MluI (3367) BsiWI (3370) PaeR7I - PspXI - XhoI (3382) PmeI (3460) PmlI (3806) AarI (3829) pLenti-C-Myc-DDK-IRES-Neo 7794 bp
BsmBI  (7219)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
PasI  (7212)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
ScaI  (7030)
1 site
A G T A C T T C A T G A
Bsu36I  (5700)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
SacII  (5481)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
BsrGI  (4860)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
SphI  (4598)
1 site
G C A T G C C G T A C G
PflFI  (4311)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (4311)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FspI  (4295)
1 site
T G C G C A A C G C G T
BclI  (4058)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BmgBI  (4033)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
SgrDI  (1)
1 site
C G T C G A C G G C A G C T G C
FseI  (1530)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
EcoNI  (1550)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
BbvCI  (1804)
1 site
C C T C A G C G G A G T C G
AleI  (1957)
1 site
C A C N N N N G T G G T G N N N N C A C
XbaI  (2613)
1 site
T