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Plasmid Files

pRSGC1-U6-sg-HTS6-CMV-Cas9-2A-Puro

Lentiviral vector for coexpressing Cas9 from the CMV promoter together with a single guide RNA (sgRNA), for permanently knocking out a target gene.

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pRSGC1-U6-sg-HTS6-CMV-Cas9-2A-Puro.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Cellecta
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SspI (10,918) PciI (9221) SfiI (8799) AanI (8570) AanI (8514) KpnI (8179) Acc65I (8175) HincII (7580) SalI (7578) SexAI * (7539) BstEII (7106) RsrII (7088) BsiWI (7028) PflFI - Tth111I (7014) BamHI (6906) NotI (6898) FseI (6877) BsaBI * (6243) MluI (1) NruI * (612) MfeI (964) BbvCI (1199) AleI (1352) BspDI - ClaI (1798) BbsI - BpiI (2064) BbsI - BpiI (2090) PaeR7I - PspXI - XhoI (2113) EcoRI (2280) AgeI (2289) BstBI (2298) SnaBI (2361) PspOMI (4100) ApaI (4104) EcoRV (4598) PmlI (5534)