Resources
Plasmid Files

RNAi-Ready pSIREN-Shuttle (linearized)

Linearized vector for expressing a small hairpin RNA (shRNA) in mammalian cells.

To obtain this DNA and protein sequence with restriction sites, please download
 SnapGene or the free  SnapGene Viewer.

pSIREN-Shuttle (linearized) Sequence and MappSIREN-Shuttle (linearized).dna
Map and Sequence File   
Sequence Author:  Clontech
Download Free Trial Get SnapGene Viewer

 < BamHI > (3140) I-CeuI (2867) < EcoRI > (0) PI-SceI (20) RNAi-Ready pSIREN-Shuttle 3140 bp
End  (3140)
0 sites
I-CeuI  (2867)
1 site
T A A C T A T A A C G G T C C T A A G G T A G C G A A T T G A T A T T G C C A G G A T T C C A T C G C T

I-CeuI is a homing endonuclease that can recognize a variety of
similar recognition sequences.
Start  (0)
0 sites
PI-SceI  (20)
1 site
A T C T A T G T C G G G T G C G G A G A A A G A G G T A A T G A A A T G G T A G A T A C A G C C C A C G C C T C T T T C T C C A T T A C T T T A C C

PI-SceI  is a homing endonuclease that can recognize a variety of
similar recognition sequences.
KanR
1583 .. 2398  =  816 bp
271 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
KanR
1583 .. 2398  =  816 bp
271 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
ori
298 .. 886  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
298 .. 886  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
U6 promoter
2897 .. 3137  =  241 bp
RNA polymerase III promoter for human U6 snRNA
U6 promoter
2897 .. 3137  =  241 bp
RNA polymerase III promoter for human U6 snRNA
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter