bleMX6

bleMX6 selector module conferring phleomycin resistance, for gene disruption in yeast.
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No matches
800 600 400 200 End (922) BsmI (909) ScaI (724) TatI (722) BclI * (717) DraIII (698) BaeGI - Bme1580I (694) ApaLI (690) EagI (676) BglI (650) FseI (621) MmeI (559) BanI (515) CsiI - SexAI * (508) SgrAI (459) SmaI (435) XmaI - TspMI (433) BsrBI (399) AatII (391) ZraI (389) MauBI - BssHII (381) BsaWI (368) HincII (355) MscI (348) StyI NcoI (343) BciVI (301) DraI (294) MluI - AflIII (203) BfuAI - BspMI (181) PstI (170) Bpu10I (48) Start (0) bleMX6 TEF promoter BleoR TEF terminator bleMX6 922 bp
End  (922)
0 sites
BsmI  (909)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
ScaI  (724)
1 site
A G T A C T T C A T G A
TatI  (722)
1 site
W G T A C W W C A T G W
BclI  (717)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
DraIII  (698)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
BaeGI  (694)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (694)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
ApaLI  (690)
1 site
G T G C A C C A C G T G
EagI  (676)
1 site
C G G C C G G C C G G C
BglI  (650)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
FseI  (621)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
MmeI  (559)
1 site
T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18

Efficient cleavage requires at least two copies of the MmeI recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BanI  (515)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
CsiI  (508)
1 site
A C C W G G T T G G W C C A

Sticky ends from different CsiI sites may not be compatible.
SexAI  (508)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
SgrAI  (459)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
SmaI  (435)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
XmaI  (433)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
TspMI  (433)
1 site
C C C G G G G G G C C C
BsrBI  (399)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
AatII  (391)
1 site
G A C G T C C T G C A G
ZraI  (389)
1 site
G A C G T C C T G C A G
MauBI  (381)
1 site
C G C G C G C G G C G C G C G C
BssHII  (381)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
BsaWI  (368)
1 site
W C C G G W W G G C C W

Cleavage may be enhanced when more than one copy of the BsaWI recognition sequence is present.
HincII  (355)
1 site
G T Y R A C C A R Y T G
MscI  (348)
1 site
T G G C C A A C C G G T
StyI  (343)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
NcoI  (343)
1 site
C C A T G G G G T A C C
BciVI  (301)
1 site
G T A T C C ( N ) 5 N C A T A G G ( N ) 5

The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
DraI  (294)
1 site
T T T A A A A A A T T T
MluI  (203)
1 site
A C G C G T T G C G C A
AflIII  (203)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
BfuAI  (181)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (181)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
PstI  (170)
1 site
C T G C A G G A C G T C
Bpu10I  (48)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
Start  (0)
0 sites
bleMX6
1 .. 922  =  922 bp
yeast selectable marker conferring phleomycin resistance
bleMX6
1 .. 922  =  922 bp
yeast selectable marker conferring phleomycin resistance
TEF promoter
1 .. 344  =  344 bp
Ashbya gossypii TEF promoter
TEF promoter
1 .. 344  =  344 bp
Ashbya gossypii TEF promoter
BleoR
345 .. 719  =  375 bp
124 amino acids  =  13.8 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and Zeocin™
BleoR
345 .. 719  =  375 bp
124 amino acids  =  13.8 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and Zeocin™
TEF terminator
725 .. 922  =  198 bp
Ashbya gossypii TEF terminator
TEF terminator
725 .. 922  =  198 bp
Ashbya gossypii TEF terminator
ORF:  345 .. 719  =  375 bp
ORF:  124 amino acids  =  13.8 kDa
ORF:  323 .. 625  =  303 bp
ORF:  100 amino acids  =  10.8 kDa
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Download bleMX6.dna file

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Individual Sequences & Maps

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