pYM25

Plasmid with a hygromycin resistance marker that provides a template for PCR to fuse yeast enhanced GFP (yeGFP) to the C-terminus of a protein of interest.

Sequence Author: EUROSCARF

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Yeast Plasmids | More Plasmid Sets
No matches
HindIII (19) PvuII (15) NotI (6) PfoI (4684) SspI (4455) XmnI (4250) BmrI (3691) PciI (2758) BspQI - SapI (2642) NotI (2521) SfiI (2520) SpeI (2507) EcoRV (2497) BspDI - ClaI (2490) BanII - SacI (2481) BsiWI (25) SalI (37) AccI (38) AarI - BamHI (43) MscI (252) MfeI (641) PflMI (777) BssHII (801) BglII (808) BstEII (838) BmgBI (891) Bpu10I (900) BseRI (1002) PshAI (1227) AsiSI (1564) RsrII (1608) BtgZI (1872) AvaI - BmeT110I - BsoBI - PaeR7I - XhoI (2227) PpuMI (2324) Eco53kI (2479) pYM25 4905 bp
HindIII  (19)
1 site
A A G C T T T T C G A A
PvuII  (15)
1 site
C A G C T G G T C G A C
NotI  (6)
2 sites
G C G G C C G C C G C C G G C G
PfoI  (4684)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
SspI  (4455)
1 site
A A T A T T T T A T A A
XmnI  (4250)
1 site
G A A N N N N T T C C T T N N N N A A G
BmrI  (3691)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
PciI  (2758)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (2642)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (2642)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
NotI  (2521)
2 sites
G C G G C C G C C G C C G G C G
SfiI  (2520)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
SpeI  (2507)
1 site
A C T A G T T G A T C A
EcoRV  (2497)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
BspDI  (2490)
1 site
A T C G A T T A G C T A
ClaI  (2490)
1 site
A T C G A T T A G C T A
BanII  (2481)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
SacI  (2481)
1 site
G A G C T C C T C G A G
BsiWI  (25)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
SalI  (37)
1 site
G T C G A C C A G C T G
AccI  (38)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
AarI  (43)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Cleavage may be enhanced when more than one copy of the AarI recognition sequence is present.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.
BamHI  (43)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
MscI  (252)
1 site
T G G C C A A C C G G T
MfeI  (641)
1 site
C A A T T G G T T A A C
PflMI  (777)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
BssHII  (801)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
BglII  (808)
1 site
A G A T C T T C T A G A
BstEII  (838)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BmgBI  (891)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
Bpu10I  (900)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BseRI  (1002)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
PshAI  (1227)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
AsiSI  (1564)
1 site
G C G A T C G C C G C T A G C G
RsrII  (1608)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BtgZI  (1872)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
AvaI  (2227)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BmeT110I  (2227)
1 site
C Y C G R G G R G C Y C
BsoBI  (2227)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
PaeR7I  (2227)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (2227)
1 site
C T C G A G G A G C T C
PpuMI  (2324)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
Eco53kI  (2479)
1 site
G A G C T C C T C G A G
HygR
1197 .. 2225  =  1029 bp
342 amino acids  =  38.1 kDa
Product: aminoglycoside phosphotransferase from E. coli
confers resistance to hygromycin
HygR
1197 .. 2225  =  1029 bp
342 amino acids  =  38.1 kDa
Product: aminoglycoside phosphotransferase from E. coli
confers resistance to hygromycin
AmpR
3578 .. 4438  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   3578 .. 4369  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3578 .. 4438  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   4370 .. 4438  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3578 .. 4438  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
yEGFP
85 .. 798  =  714 bp
237 amino acids  =  26.7 kDa
Product: yeast-enhanced green fluorescent protein
codons optimized for translation in S. cerevisiae and C. albicans
yEGFP
85 .. 798  =  714 bp
237 amino acids  =  26.7 kDa
Product: yeast-enhanced green fluorescent protein
codons optimized for translation in S. cerevisiae and C. albicans
ori
2819 .. 3407  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2819 .. 3407  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
TEF promoter
853 .. 1196  =  344 bp
Ashbya gossypii TEF promoter
TEF promoter
853 .. 1196  =  344 bp
Ashbya gossypii TEF promoter
CYC1 terminator
2279 .. 2468  =  190 bp
transcription terminator for CYC1
CYC1 terminator
2279 .. 2468  =  190 bp
transcription terminator for CYC1
AmpR promoter
4439 .. 4543  =  105 bp
AmpR promoter
4439 .. 4543  =  105 bp
SP6 promoter
4889 .. 2  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
SP6 promoter
4889 .. 2  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
ORF:  313 .. 798  =  486 bp
ORF:  161 amino acids  =  18.5 kDa
ORF:  773 .. 1063  =  291 bp
ORF:  96 amino acids  =  11.0 kDa
ORF:  1197 .. 2225  =  1029 bp
ORF:  342 amino acids  =  38.1 kDa
ORF:  3708 .. 3974  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  1170 .. 1958  =  789 bp
ORF:  262 amino acids  =  29.4 kDa
ORF:  3578 .. 4438  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
Click here to try SnapGene

Download pYM25.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.