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Plasmid Files

T5 promoter

Bacteriophage T5 promoter for E. coli RNA polymerase, with an embedded lac operator.

 
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 40 35 30 25 20 15 10 5 -35 -10 T5 promoter End (45) PsiI (40) BsrBI (27) ApoI (8) Start (0) T5 promoter 45 bp
End  (45)
0 sites
PsiI  (40)
1 site
T T A T A A A A T A T T
BsrBI  (27)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures
up to 50°C.
ApoI  (8)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
Start  (0)
0 sites
T5 promoter
1 .. 45  =  45 bp
   Segment 1:  
   1 .. 15  =  15 bp
bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator
T5 promoter
1 .. 45  =  45 bp
   Segment 2:  -35  
   16 .. 21  =  6 bp
bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator
T5 promoter
1 .. 45  =  45 bp
   Segment 3:  
   22 .. 38  =  17 bp
bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator
T5 promoter
1 .. 45  =  45 bp
   Segment 4:  -10  
   39 .. 45  =  7 bp
bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator
T5 promoter
1 .. 45  =  45 bp
4 segments
bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator
lac operator
21 .. 37  =  17 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
21 .. 37  =  17 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
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