This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
EciI (185) 1 site
Sticky ends from different EciI sites may not be compatible.
BsgI (112) 1 site
Efficient cleavage requires at least two copies of the BsgI recognition sequence.Sticky ends from different BsgI sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM).
BtgZI (111) 1 site
Sticky ends from different BtgZI sites may not be compatible.After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.BtgZI is typically used at 60°C, but is 75% active at 37°C.
BanII (12) 1 site
Sticky ends from different BanII sites may not be compatible.
Bsp1286I (12) 1 site
Sticky ends from different Bsp1286I sites may not be compatible.