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chicken β-actin promoter

Chicken beta-actin promoter.

 
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 250 200 150 100 50 chicken β- actin promoter End (278) BsrBI (199) EciI (185) BsgI (112) BtgZI (111) BanII - Bsp1286I (12) Start (0) chicken β- actin promoter 278 bp
End  (278)
0 sites
BsrBI  (199)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures
up to 50°C.
EciI  (185)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
BsgI  (112)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI
recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BtgZI  (111)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BanII  (12)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
Bsp1286I  (12)
1 site
G D G C H C C H C G D G

Sticky ends from different Bsp1286I sites may not be compatible.
Start  (0)
0 sites
chicken β-actin promoter
1 .. 278  =  278 bp
chicken β-actin promoter
1 .. 278  =  278 bp
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