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Plasmid Files

pMMB207

Broad-host-range bacterial cloning vector with the pUC18/19 MCS and a chloramphenicol resistance gene. See also pMMB208.

 
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pMMB207.dna
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HindIII (9046) SphI (9044) PstI - SbfI (9038) SfcI (9034) SalI (9028) XbaI (9022) BamHI (9016) SmaI (9013) KpnI - TspMI - XmaI (9011) Acc65I (9007) SacI (9005) Eco53kI (9003) EcoRI (8995) MauBI (8720) MluI (8263) ApaI (8060) PspOMI (8056) HpaI (7761) BspQI - SapI (7455) FspAI (6984) EcoNI (6834) PfoI * (6694) SgrAI (4746) XmnI (0) BsaI (148) BssS α I (612) BspEI (1256) SnaBI (1266) SfiI (1886) EcoO109I (2438) BstZ17I (3261) pMMB207 9076 bp
HindIII  (9046)
1 site
A A G C T T T T C G A A
SphI  (9044)
1 site
G C A T G C C G T A C G
PstI  (9038)
1 site
C T G C A G G A C G T C
SbfI  (9038)
1 site
C C T G C A G G G G A C G T C C
SfcI  (9034)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
SalI  (9028)
1 site
G T C G A C C A G C T G
XbaI  (9022)
1 site
T C T A G A A G A T C T
BamHI  (9016)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SmaI  (9013)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
KpnI  (9011)
1 site
G G T A C C C C A T G G
TspMI  (9011)
1 site
C C C G G G G G G C C C
XmaI  (9011)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
Acc65I  (9007)
1 site
G G T A C C C C A T G G
SacI  (9005)
1 site
G A G C T C C T C G A G
Eco53kI  (9003)
1 site
G A G C T C C T C G A G
EcoRI  (8995)
1 site
G A A T T C C T T A A G
MauBI  (8720)
1 site
C G C G C G C G G C G C G C G C
MluI  (8263)
1 site
A C G C G T T G C G C A
ApaI  (8060)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PspOMI  (8056)
1 site
G G G C C C C C C G G G
HpaI  (7761)
1 site
G T T A A C C A A T T G
BspQI  (7455)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (7455)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
FspAI  (6984)
1 site
R T G C G C A Y Y A C G C G T R
EcoNI  (6834)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
PfoI  (6694)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
SgrAI  (4746)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
XmnI  (0)
1 site
G A A N N N N T T C C T T N N N N A A G
BsaI  (148)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BssSαI  (612)
1 site
C A C G A G G T G C T C
BspEI  (1256)
1 site
T C C G G A A G G C C T
SnaBI  (1266)
1 site
T A C G T A A T G C A T
SfiI  (1886)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
EcoO109I  (2438)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BstZ17I  (3261)
1 site
G T A T A C C A T A T G
lacI
7536 .. 8618  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lacI
7536 .. 8618  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
RSF1010 RepB
4100 .. 5071  =  972 bp
323 amino acids  =  35.9 kDa
Product: replication protein B of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
RSF1010 RepB
4100 .. 5071  =  972 bp
323 amino acids  =  35.9 kDa
Product: replication protein B of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
RSF1010 RepC
6408 .. 7259  =  852 bp
283 amino acids  =  31.1 kDa
Product: replication protein C of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
RSF1010 RepC
6408 .. 7259  =  852 bp
283 amino acids  =  31.1 kDa
Product: replication protein C of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
CmR
1047 .. 1706  =  660 bp
219 amino acids  =  25.6 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
1047 .. 1706  =  660 bp
219 amino acids  =  25.6 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
RSF1010 oriV
2038 .. 2432  =  395 bp
replication origin of the broad-host-range plasmid RSF1010; requires the RSF1010 RepA/B/C proteins for replication (Scholz et al., 1989)
RSF1010 oriV
2038 .. 2432  =  395 bp
replication origin of the broad-host-range plasmid RSF1010; requires the RSF1010 RepA/B/C proteins for replication (Scholz et al., 1989)
cat promoter
944 .. 1046  =  103 bp
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
cat promoter
944 .. 1046  =  103 bp
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
RSF1010 oriT
2774 .. 2861  =  88 bp
origin of transfer of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
RSF1010 oriT
2774 .. 2861  =  88 bp
origin of transfer of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
rrnB T1 terminator
178 .. 264  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
178 .. 264  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
lacIq promoter
8619 .. 8696  =  78 bp
In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold.
lacIq promoter
8619 .. 8696  =  78 bp
In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold.
MCS
8995 .. 9051  =  57 bp
pUC18/19 multiple cloning site
MCS
8995 .. 9051  =  57 bp
pUC18/19 multiple cloning site
tac promoter
8926 .. 8954  =  29 bp
   Segment 1:  -35  
   8926 .. 8931  =  6 bp
strong E. coli promoter; hybrid between the trp and lac UV5 promoters
tac promoter
8926 .. 8954  =  29 bp
   Segment 2:  
   8932 .. 8947  =  16 bp
strong E. coli promoter; hybrid between the trp and lac UV5 promoters
tac promoter
8926 .. 8954  =  29 bp
   Segment 3:  -10  
   8948 .. 8954  =  7 bp
strong E. coli promoter; hybrid between the trp and lac UV5 promoters
tac promoter
8926 .. 8954  =  29 bp
3 segments
strong E. coli promoter; hybrid between the trp and lac UV5 promoters
rrnB T2 terminator
356 .. 383  =  28 bp
transcription terminator T2 from the E. coli rrnB gene
rrnB T2 terminator
356 .. 383  =  28 bp
transcription terminator T2 from the E. coli rrnB gene
lac operator
8962 .. 8978  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
8962 .. 8978  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
RSF1010 RepA
5582 .. 6421  =  840 bp
279 amino acids  =  29.9 kDa
Product: replication protein A of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
RSF1010 RepA
5582 .. 6421  =  840 bp
279 amino acids  =  29.9 kDa
Product: replication protein A of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
ORF:  1624 .. 2031  =  408 bp
ORF:  135 amino acids  =  15.0 kDa
ORF:  494 .. 727  =  234 bp
ORF:  77 amino acids  =  8.9 kDa
ORF:  2942 .. 5071  =  2130 bp
ORF:  709 amino acids  =  78.0 kDa
ORF:  5582 .. 6421  =  840 bp
ORF:  279 amino acids  =  29.9 kDa
ORF:  1047 .. 1706  =  660 bp
ORF:  219 amino acids  =  25.6 kDa
ORF:  2238 .. 2666  =  429 bp
ORF:  142 amino acids  =  15.3 kDa
ORF:  2784 .. 3191  =  408 bp
ORF:  135 amino acids  =  14.3 kDa
ORF:  3690 .. 4103  =  414 bp
ORF:  137 amino acids  =  15.1 kDa
ORF:  4527 .. 5171  =  645 bp
ORF:  214 amino acids  =  22.8 kDa
ORF:  6309 .. 7259  =  951 bp
ORF:  316 amino acids  =  34.4 kDa
ORF:  8937 .. 97  =  237 bp
ORF:  78 amino acids  =  8.5 kDa
ORF:  425 .. 793  =  369 bp
ORF:  122 amino acids  =  14.1 kDa
ORF:  2459 .. 2743  =  285 bp
ORF:  94 amino acids  =  10.9 kDa
ORF:  2813 .. 3469  =  657 bp
ORF:  218 amino acids  =  22.9 kDa
ORF:  3662 .. 4000  =  339 bp
ORF:  112 amino acids  =  12.0 kDa
ORF:  4217 .. 4810  =  594 bp
ORF:  197 amino acids  =  20.5 kDa
ORF:  5579 .. 6346  =  768 bp
ORF:  255 amino acids  =  27.0 kDa
ORF:  6749 .. 7090  =  342 bp
ORF:  113 amino acids  =  12.6 kDa
ORF:  19 .. 345  =  327 bp
ORF:  108 amino acids  =  12.1 kDa
ORF:  1660 .. 1905  =  246 bp
ORF:  81 amino acids  =  9.4 kDa
ORF:  3109 .. 3339  =  231 bp
ORF:  76 amino acids  =  8.4 kDa
ORF:  4006 .. 4359  =  354 bp
ORF:  117 amino acids  =  12.6 kDa
ORF:  5833 .. 6198  =  366 bp
ORF:  121 amino acids  =  13.3 kDa
ORF:  7516 .. 7779  =  264 bp
ORF:  87 amino acids  =  8.9 kDa
ORF:  8368 .. 8685  =  318 bp
ORF:  105 amino acids  =  11.2 kDa
ORF:  1668 .. 1898  =  231 bp
ORF:  76 amino acids  =  8.5 kDa
ORF:  6642 .. 6980  =  339 bp
ORF:  112 amino acids  =  12.1 kDa
ORF:  7536 .. 8495  =  960 bp
ORF:  319 amino acids  =  34.1 kDa
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