Resources
Plasmid Files

MLM3636

CRISPR/Cas9 plasmid for expressing a guide RNA (gRNA) from the U6 promoter.

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MLM3636 Sequence and MapMLM3636.dna
Map and Sequence File   
Sequence Author:  Joung Lab / Addgene #43860
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 BsmBI (325) BsmBI (350) MLM3636 2278 bp
BsmBI  (325)
2 sites
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI  (350)
2 sites
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
AmpR
1288 .. 2148  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   1288 .. 2079  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
1288 .. 2148  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   2080 .. 2148  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
1288 .. 2148  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
ori
529 .. 1117  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
529 .. 1117  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
U6 promoter
82 .. 322  =  241 bp
RNA polymerase III promoter for human U6 snRNA
U6 promoter
82 .. 322  =  241 bp
RNA polymerase III promoter for human U6 snRNA
AmpR promoter
2149 .. 2253  =  105 bp
AmpR promoter
2149 .. 2253  =  105 bp
gRNA scaffold
350 .. 425  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system
gRNA scaffold
350 .. 425  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system
polIII terminator
426 .. 431  =  6 bp
RNA polymerase III transcription terminator
polIII terminator
426 .. 431  =  6 bp
RNA polymerase III transcription terminator
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