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Plasmid Files

SP-dCas9-VPR

Plasmid for mammalian cell expression of catalytically inactive Cas9m4 fused to the tripartite VP64-p65-Rta (VPR) transcriptional activator.

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SP-dCas9-VPR.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Church Lab / Addgene #63798
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MfeI (11,295) SgrDI (11,132) AhdI (10,211) BstZ17I (8939) RsrII (8481) AvrII (7755) SexAI * (7522) DraIII (7232) PmeI (6687) KpnI (6600) Acc65I (6596) NotI (6588) BbvCI (6191) PshAI (5898) SpeI (64) SnaBI (405) SbfI (995) BlpI (1696) PmlI (3537) FspAI (3698) AarI (4008) PaeR7I - XhoI (4291) EcoRI (4570) AscI (4703)