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Plasmid Files

pST1374-N-NLS-flag-linker-Cas9-H840A

Plasmid for mammalian cell expression of Cas9 with the H840A nickase mutation and with an N-terminal nuclear localization signal followed by a FLAG® tag.

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pST1374-N-NLS-flag-linker-Cas9-H840A.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Huang Lab / Addgene #51129
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SgrDI (9316) Acc16I - AviII - FspI - NsbI (8617) AhdI - AspEI - BmeRI - DriI - Eam1105I (8395) BssNAI - Bst1107I - BstZ17I (7123) AspI - PflFI - PsyI - Tth111I (6709) BalI - MlsI - MluNI - MscI - Msp20I (6438) SmaI (6329) Cfr9I - TspMI - XmaI - XmaCI (6327) AspA2I - AvrII - BlnI - XmaJI (6306) AatI - Eco147I - PceI - SseBI - StuI (6305) CsiI - MabI - SexAI * (6073) MssI - PmeI (5257) AgeI - AsiGI - BshTI - CspAI - PinAI (5227) ApaI (5174) Bsp120I - PspOMI (5170) MfeI - MunI (161) AhlI - BcuI - SpeI (249) BstSNI - Eco105I - SnaBI (590) Ecl136II - Eco53kI - EcoICRI (816) Psp124BI - SacI - SstI (818) AsuNHI - NheI (895) BmtI - BspOI (899) CciNI - NotI (932) XbaI (964) AccB7I - PflMI - Van91I (1006) CpoI - CspI - RsrII - Rsr2I (1259) AxyI - Bse21I - Bsu36I - Eco81I (1513) PaeR7I - Sfr274I - SlaI - XhoI (2200) BbvCI (2257) BamHI (2402) AjiI - BmgBI - BtrI (3573) BstXI (3852) KflI - SanDI (4440)