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Plasmid Files

pTPGI_dCas9_VP64

Yeast plasmid for inducible expression of catalytically inactive S. pyogenes dCas9 fused to the VP64 transcriptional activation domain.

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pTPGI_dCas9_VP64.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Lu Lab / Addgene #49013
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ZraI (10,920) AhdI (10,000) PciI (9107) KpnI (8713) Acc65I (8709) MluI (8628) BsrGI (8621) PaeR7I - PspXI - XhoI (8450) AvrII (8442) SalI (8273) KflI (7528) AatII (10,922) PfoI (46) BspEI * (332) PmlI (427) MfeI (607) Bsu36I (709) EcoRV (752) AarI (900) HindIII (980) NgoMIV (1566) NaeI (1568) PacI (2078) Eco53kI (2084) SacI (2086) AflII (2087) NdeI (2129) AfeI (2306) NsiI (2325) NruI (2430) EcoNI (2501) SpeI (2719) EagI - NotI (3134) NcoI (3157) PspOMI * (3326) ApaI * (3330) StuI (3388) EcoRI (3631) BseRI (3680) AgeI (3712) PstI (3914) BamHI (4094) TspMI - XmaI (4148) SmaI (4150) SexAI * (4837)