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Plasmid Files

FusionRed

Monomeric red fluorescent protein optimized for use as a fusion tag.

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FusionRed Sequence and MapFusionRed.dna
Map and Sequence File   
Sequence Author:  Evrogen
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 600 400 200 End (699) HaeII (680) MscI (658) EaeI (656) DraIII (648) PshAI (631) PflMI (597) AccI (578) BsaHI (575) BstYI (538) AcuI (508) BglI (467) MspA1I (451) BsmBI (430) StuI (427) NmeAIII (396) PfoI * (332) BpmI (316) BbsI (299) Bsu36I (257) EcoP15I (228) TsoI (166) TaqII (130) BsiHKAI (82) ApaLI (78) BsrGI - TatI (41) MslI (31) Start (0) FusionRed 699 bp
End  (699)
0 sites
HaeII  (680)
1 site
R G C G C Y Y C G C G R
MscI  (658)
1 site
T G G C C A A C C G G T
EaeI  (656)
1 site
Y G G C C R R C C G G Y
DraIII  (648)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PshAI  (631)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
PflMI  (597)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
AccI  (578)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the
recognition sequence.
Sticky ends from different AccI sites may not be compatible.
BsaHI  (575)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
BstYI  (538)
1 site
R G A T C Y Y C T A G R
AcuI  (508)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the AcuI
recognition sequence.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BglI  (467)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
MspA1I  (451)
1 site
C M G C K G G K C G M C
BsmBI  (430)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
StuI  (427)
1 site
A G G C C T T C C G G A
NmeAIII  (396)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII
recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PfoI  (332)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
BpmI  (316)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI
recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BbsI  (299)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
Bsu36I  (257)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
EcoP15I  (228)
1 site
C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N

Efficient cleavage requires two inversely oriented copies of the
EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
TsoI  (166)
1 site
T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9

Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
TaqII  (130)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
BsiHKAI  (82)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
ApaLI  (78)
1 site
G T G C A C C A C G T G
BsrGI  (41)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
TatI  (41)
1 site
W G T A C W W C A T G W
MslI  (31)
1 site
C A Y N N N N R T G G T R N N N N Y A C
Start  (0)
0 sites
FusionRed
1 .. 699  =  699 bp
232 amino acids  =  25.6 kDa
Product: monomeric red fluorescent protein
optimized for use as a fusion tag (Shemiakina et al.,
2012)
mammalian codon-optimized
FusionRed
1 .. 699  =  699 bp
232 amino acids  =  25.6 kDa
Product: monomeric red fluorescent protein
optimized for use as a fusion tag (Shemiakina et al.,
2012)
mammalian codon-optimized
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