Resources
Plasmid Files

IFP2.0

Monomeric infrared fluorescent protein derived from a bacterial phytochrome, engineered for improved brightness relative to IFP1.4.

 
To obtain this DNA and protein sequence with restriction sites, please download
 SnapGene or the free  SnapGene Viewer.

 800 600 400 200 End (966) SbfI (944) BsaAI (885) AhdI (859) MspA1I - PvuII (846) Bsu36I (790) EciI (761) ApaI (642) PspOMI (638) SphI (585) BsrGI - TatI (524) BsiEI (513) AcuI - PfoI (491) BsrDI (434) NmeAIII (380) ApoI - EcoRI (373) BsaHI (284) EcoNI (187) SexAI * (93) BsrBI (75) KflI - PpuMI (9) Start (0) IFP2.0 966 bp
End  (966)
0 sites
SbfI  (944)
1 site
C C T G C A G G G G A C G T C C
BsaAI  (885)
1 site
Y A C G T R R T G C A Y
AhdI  (859)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
MspA1I  (846)
1 site
C M G C K G G K C G M C
PvuII  (846)
1 site
C A G C T G G T C G A C
Bsu36I  (790)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
EciI  (761)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
ApaI  (642)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PspOMI  (638)
1 site
G G G C C C C C C G G G
SphI  (585)
1 site
G C A T G C C G T A C G
BsrGI  (524)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
TatI  (524)
1 site
W G T A C W W C A T G W
BsiEI  (513)
1 site
C G R Y C G G C Y R G C

Sticky ends from different BsiEI sites may not be compatible.
AcuI  (491)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the AcuI
recognition sequence.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
PfoI  (491)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
BsrDI  (434)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
NmeAIII  (380)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII
recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
ApoI  (373)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
EcoRI  (373)
1 site
G A A T T C C T T A A G
BsaHI  (284)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
EcoNI  (187)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
SexAI  (93)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
BsrBI  (75)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures
up to 50°C.
KflI  (9)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
PpuMI  (9)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
Start  (0)
0 sites
IFP2.0
1 .. 966  =  966 bp
321 amino acids  =  34.9 kDa
Product: bacteriophytochrome-based monomeric
infrared fluorescent protein (Yu et al., 2014)
IFP2.0
1 .. 966  =  966 bp
321 amino acids  =  34.9 kDa
Product: bacteriophytochrome-based monomeric
infrared fluorescent protein (Yu et al., 2014)
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter