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Plasmid Files

SYFP2

Monomeric optimized variant of EYFP, identical to mVenus but with L68V.

 
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SYFP2.dna
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600 400 200 SYFP2 End (717) BsrGI (710) BlpI (622) Bpu10I (604) BstYI (500) PfoI * (281) Bts α I (214) PasI (192) BssS α I (181) BsrFI (151) BtgZI (122) EaeI (72) BanI (36) BseRI (31) Start (0) SYFP2 717 bp
End  (717)
0 sites
BsrGI  (710)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
BlpI  (622)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
Bpu10I  (604)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BstYI  (500)
1 site
R G A T C Y Y C T A G R
PfoI  (281)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
BtsαI  (214)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsαI sites may not be compatible.
PasI  (192)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
BssSαI  (181)
1 site
C A C G A G G T G C T C
BsrFI  (151)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI  (122)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
EaeI  (72)
1 site
Y G G C C R R C C G G Y
BanI  (36)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
BseRI  (31)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
Start  (0)
0 sites
SYFP2
1 .. 717  =  717 bp
239 amino acids  =  26.9 kDa
   Segment 1:  
   1 .. 3  =  3 bp
   1 amino acid  =  149.2 Da
Product: monomeric optimized variant of EYFP, identical to mVenus but with L68V (Kremers et al., 2006)
mammalian codon-optimized
SYFP2
1 .. 717  =  717 bp
239 amino acids  =  26.9 kDa
   Segment 2:  1a  
   4 .. 6  =  3 bp
   1 amino acid  =  117.2 Da
Product: monomeric optimized variant of EYFP, identical to mVenus but with L68V (Kremers et al., 2006)
mammalian codon-optimized
SYFP2
1 .. 717  =  717 bp
239 amino acids  =  26.9 kDa
   Segment 3:  
   7 .. 717  =  711 bp
   237 amino acids  =  26.6 kDa
Product: monomeric optimized variant of EYFP, identical to mVenus but with L68V (Kremers et al., 2006)
mammalian codon-optimized
SYFP2
1 .. 717  =  717 bp
239 amino acids  =  26.9 kDa
3 segments
Product: monomeric optimized variant of EYFP, identical to mVenus but with L68V (Kremers et al., 2006)
mammalian codon-optimized
ORF:  1 .. 717  =  717 bp
ORF:  239 amino acids  =  26.9 kDa
ORF:  3 .. 716  =  714 bp
ORF:  238 amino acids  =  27.5 kDa  (no start codon)
ORF:  1 .. 717  =  717 bp
ORF:  239 amino acids  =  23.9 kDa  (no start codon)
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