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Plasmid Files

TurboGFP

Improved green fluorescent protein from Pontellina plumata.

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TurboGFP Sequence and MapTurboGFP.dna
Map and Sequence File   
Sequence Author:  Evrogen
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 600 400 200 TurboGFP End (699) BseRI (628) ApaI - BanII (589) PspOMI (585) PstI (578) NspI (543) BsaBI * (464) AarI (452) BsiEI (345) FseI (344) EaeI - EagI - NaeI (342) NgoMIV (340) XmnI (238) MspA1I (224) PflMI (186) BlpI (175) PluTI (152) SfoI (150) BsaHI - NarI (149) KasI (148) EcoP15I (144) BspQI - EarI - SapI (130) BsrBI (17) Start (0) TurboGFP 699 bp
End  (699)
0 sites
BseRI  (628)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
ApaI  (589)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
BanII  (589)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
PspOMI  (585)
1 site
G G G C C C C C C G G G
PstI  (578)
1 site
C T G C A G G A C G T C
NspI  (543)
1 site
R C A T G Y Y G T A C R
BsaBI  (464)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
AarI  (452)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI
recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its
electrophoretic mobility.
BsiEI  (345)
1 site
C G R Y C G G C Y R G C

Sticky ends from different BsiEI sites may not be compatible.
FseI  (344)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
EaeI  (342)
1 site
Y G G C C R R C C G G Y
EagI  (342)
1 site
C G G C C G G C C G G C
NaeI  (342)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI
recognition sequence.
NgoMIV  (340)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV
recognition sequence.
XmnI  (238)
1 site
G A A N N N N T T C C T T N N N N A A G
MspA1I  (224)
1 site
C M G C K G G K C G M C
PflMI  (186)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
BlpI  (175)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
PluTI  (152)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
SfoI  (150)
1 site
G G C G C C C C G C G G
BsaHI  (149)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
NarI  (149)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
KasI  (148)
1 site
G G C G C C C C G C G G
EcoP15I  (144)
1 site
C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N

Efficient cleavage requires two inversely oriented copies of the
EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
BspQI  (130)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
EarI  (130)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI
recognition sequence.
Sticky ends from different EarI sites may not be compatible.
SapI  (130)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be
mixed before removing an aliquot.
BsrBI  (17)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures
up to 50°C.
Start  (0)
0 sites
TurboGFP
1 .. 699  =  699 bp
232 amino acids  =  25.7 kDa
Product: improved green fluorescent protein from
Pontellina plumata (Evdokimov et al., 2006)
mammalian codon-optimized
TurboGFP
1 .. 699  =  699 bp
232 amino acids  =  25.7 kDa
Product: improved green fluorescent protein from
Pontellina plumata (Evdokimov et al., 2006)
mammalian codon-optimized
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