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Plasmid Files

dTomato

Dimeric variant of DsRed fluorescent protein.

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dTomato Sequence and MapdTomato.dna
Map and Sequence File   
Sequence Author:  Tsien Lab
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 600 400 200 End (702) BsrGI - TatI (695) AfeI (649) AleI (631) MscI (550) BstXI (546) SexAI * (521) AlwNI * (495) EcoO109I * (492) BstYI (482) StuI (436) PspFI (432) BseYI (428) BbsI (424) BtgI - NcoI (422) PstI - SbfI (344) SfcI (340) PshAI (308) BmeT110I (266) AvaI - BsoBI (265) BsiWI (215) BmrI (186) AhdI (183) TaqII (139) BaeGI - Bme1580I (124) BssS α I (76) BanII (64) FspI - FspAI (52) Start (0) dTomato 702 bp
End  (702)
0 sites
BsrGI  (695)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
TatI  (695)
1 site
W G T A C W W C A T G W
AfeI  (649)
1 site
A G C G C T T C G C G A
AleI  (631)
1 site
C A C N N N N G T G G T G N N N N C A C
MscI  (550)
1 site
T G G C C A A C C G G T
BstXI  (546)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
SexAI  (521)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
AlwNI  (495)
1 site
C A G N N N C T G G T C N N N G A C
* Blocked by Dcm methylation.
Sticky ends from different AlwNI sites may not be compatible.
EcoO109I  (492)
1 site
R G G N C C Y Y C C N G G R
* Blocked by Dcm methylation.
Sticky ends from different EcoO109I sites may not be compatible.
BstYI  (482)
1 site
R G A T C Y Y C T A G R
StuI  (436)
1 site
A G G C C T T C C G G A
PspFI  (432)
1 site
C C C A G C G G G T C G
BseYI  (428)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
BbsI  (424)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BtgI  (422)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (422)
1 site
C C A T G G G G T A C C
PstI  (344)
1 site
C T G C A G G A C G T C
SbfI  (344)
1 site
C C T G C A G G G G A C G T C C
SfcI  (340)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long
incubations.
PshAI  (308)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
BmeT110I  (266)
1 site
C Y C G R G G R G C Y C
AvaI  (265)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (265)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures
up to 65°C.
BsiWI  (215)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
BmrI  (186)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the
absence of magnesium.
AhdI  (183)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
TaqII  (139)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
BaeGI  (124)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (124)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
BssSαI  (76)
1 site
C A C G A G G T G C T C
BanII  (64)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
FspI  (52)
1 site
T G C G C A A C G C G T
FspAI  (52)
1 site
R T G C G C A Y Y A C G C G T R
Start  (0)
0 sites
dTomato
1 .. 702  =  702 bp
234 amino acids  =  27.0 kDa
Product: dimeric variant of DsRed fluorescent
protein (Shaner et al., 2004)
mammalian codon-optimized
dTomato
1 .. 702  =  702 bp
234 amino acids  =  27.0 kDa
Product: dimeric variant of DsRed fluorescent
protein (Shaner et al., 2004)
mammalian codon-optimized
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