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Plasmid Files

hmKeima-Red

Humanized monomeric Keima-Red fluorescent protein.

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hmKeima-Red.dna
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Sequence Author:  MBL International
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600 400 200 hmKeima-Red End (669) BglI (662) BsaBI * - Bse8I - BseJI * - MamI * (640) Bts α I (634) BtgZI (627) AleI - MslI - OliI - RseI - SmiMI (619) AjiI - BmgBI - BtrI (591) BauI - BssS α I - Bst2BI (568) BseX3I - BstZI - EagI - EclXI - Eco52I (561) AcuI - Eco57I (505) BsaMI - BsmI - Mva1269I - PctI (461) BbsI - BpiI - BpuAI - BstV2I (412) PasI (411) MspA1I (365) BaeGI - Bme1580I - BseSI - BstSLI (307) Alw44I - ApaLI - VneI (303) BbeI - PluTI (299) DinI - EgeI - EheI - SfoI (297) AcyI - BsaHI - BssNI - BstACI - Hin1I - Hsp92I - Mly113I - NarI (296) KasI - SspDI (295) Bpu10I (172) BssT1I - Eco130I - EcoT14I - ErhI - StyI (137) AccBSI - BsrBI - MbiI (47) Bsp1407I - BsrGI - BstAUI - SspBI - TatI (38) Start (0) hmKeima-Red 669 bp
End  (669)
0 sites
BglI  (662)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BsaBI  (640)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
Bse8I  (640)
1 site
G A T N N N N A T C C T A N N N N T A G
BseJI  (640)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
MamI  (640)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
BtsαI  (634)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsαI sites may not be compatible.
BtgZI  (627)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
AleI  (619)
1 site
C A C N N N N G T G G T G N N N N C A C
MslI  (619)
1 site
C A Y N N N N R T G G T R N N N N Y A C
OliI  (619)
1 site
C A C N N N N G T G G T G N N N N C A C
RseI  (619)
1 site
C A Y N N N N R T G G T R N N N N Y A C
SmiMI  (619)
1 site
C A Y N N N N R T G G T R N N N N Y A C
AjiI  (591)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by AjiI will not always regenerate an AjiI site.
BmgBI  (591)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
BtrI  (591)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BtrI will not always regenerate a BtrI site.
BauI  (568)
1 site
C A C G A G G T G C T C
BssSαI  (568)
1 site
C A C G A G G T G C T C
Bst2BI  (568)
1 site
C A C G A G G T G C T C
BseX3I  (561)
1 site
C G G C C G G C C G G C
BstZI  (561)
1 site
C G G C C G G C C G G C
EagI  (561)
1 site
C G G C C G G C C G G C
EclXI  (561)
1 site
C G G C C G G C C G G C
Eco52I  (561)
1 site
C G G C C G G C C G G C
AcuI  (505)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the AcuI recognition sequence.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
Eco57I  (505)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the Eco57I recognition sequence.
Sticky ends from different Eco57I sites may not be compatible.
After cleavage, Eco57I can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BsaMI  (461)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsaMI sites may not be compatible.
BsmI  (461)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
Mva1269I  (461)
1 site
G A A T G C N C T T A C G N

Sticky ends from different Mva1269I sites may not be compatible.
PctI  (461)
1 site
G A A T G C N C T T A C G N

Sticky ends from different PctI sites may not be compatible.
BbsI  (412)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BpiI  (412)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BpiI sites may not be compatible.
BpuAI  (412)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BpuAI sites may not be compatible.
BstV2I  (412)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BstV2I sites may not be compatible.
PasI  (411)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
MspA1I  (365)
1 site
C M G C K G G K C G M C
BaeGI  (307)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (307)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
BseSI  (307)
1 site
G K G C M C C M C G K G

Sticky ends from different BseSI sites may not be compatible.
BstSLI  (307)
1 site
G K G C M C C M C G K G

Sticky ends from different BstSLI sites may not be compatible.
Alw44I  (303)
1 site
G T G C A C C A C G T G
ApaLI  (303)
1 site
G T G C A C C A C G T G
VneI  (303)
1 site
G T G C A C C A C G T G
BbeI  (299)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the BbeI recognition sequence.
PluTI  (299)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
DinI  (297)
1 site
G G C G C C C C G C G G
EgeI  (297)
1 site
G G C G C C C C G C G G
EheI  (297)
1 site
G G C G C C C C G C G G
SfoI  (297)
1 site
G G C G C C C C G C G G
AcyI  (296)
1 site
G R C G Y C C Y G C R G
BsaHI  (296)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
BssNI  (296)
1 site
G R C G Y C C Y G C R G
BstACI  (296)
1 site
G R C G Y C C Y G C R G
Hin1I  (296)
1 site
G R C G Y C C Y G C R G
Hsp92I  (296)
1 site
G R C G Y C C Y G C R G
Mly113I  (296)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the Mly113I recognition sequence.
NarI  (296)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (295)
1 site
G G C G C C C C G C G G
SspDI  (295)
1 site
G G C G C C C C G C G G
Bpu10I  (172)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BssT1I  (137)
1 site
C C W W G G G G W W C C

Sticky ends from different BssT1I sites may not be compatible.
Eco130I  (137)
1 site
C C W W G G G G W W C C

Sticky ends from different Eco130I sites may not be compatible.
EcoT14I  (137)
1 site
C C W W G G G G W W C C

Sticky ends from different EcoT14I sites may not be compatible.
ErhI  (137)
1 site
C C W W G G G G W W C C

Sticky ends from different ErhI sites may not be compatible.
StyI  (137)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
AccBSI  (47)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends generated by AccBSI will not always regenerate an AccBSI site.
BsrBI  (47)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
MbiI  (47)
1 site
G A G C G G C T C G C C

This recognition sequence is asymmetric, so ligating blunt ends generated by MbiI will not always regenerate an MbiI site.
Bsp1407I  (38)
1 site
T G T A C A A C A T G T
BsrGI  (38)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
BstAUI  (38)
1 site
T G T A C A A C A T G T
SspBI  (38)
1 site
T G T A C A A C A T G T
TatI  (38)
1 site
W G T A C W W C A T G W
Start  (0)
0 sites
hmKeima-Red
1 .. 669  =  669 bp
222 amino acids  =  25.1 kDa
   Segment 1:  
   1 .. 3  =  3 bp
   1 amino acid  =  149.2 Da
Product: humanized monomeric Keima-Red fluorescent protein
mammalian codon-optimized
hmKeima-Red
1 .. 669  =  669 bp
222 amino acids  =  25.1 kDa
   Segment 2:  1a  
   4 .. 6  =  3 bp
   1 amino acid  =  117.2 Da
Product: humanized monomeric Keima-Red fluorescent protein
mammalian codon-optimized
hmKeima-Red
1 .. 669  =  669 bp
222 amino acids  =  25.1 kDa
   Segment 3:  
   7 .. 669  =  663 bp
   220 amino acids  =  24.8 kDa
Product: humanized monomeric Keima-Red fluorescent protein
mammalian codon-optimized
hmKeima-Red
1 .. 669  =  669 bp
222 amino acids  =  25.1 kDa
3 segments
Product: humanized monomeric Keima-Red fluorescent protein
mammalian codon-optimized
ORF:  1 .. 669  =  669 bp
ORF:  222 amino acids  =  25.1 kDa
ORF:  3 .. 668  =  666 bp
ORF:  222 amino acids  =  25.7 kDa  (no start codon)
ORF:  1 .. 669  =  669 bp
ORF:  223 amino acids  =  21.6 kDa  (no start codon)
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