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Plasmid Files

hmUkG1

Humanized monomeric variant of Umikinoko-Green fluorescent protein.

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hmUkG1 Sequence and MaphmUkG1.dna
Map and Sequence File   
Sequence Author:  MBL International
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 600 400 200 hmUkG1 End (684) BtgI (641) BsiEI (606) Bpu10I (595) BstXI (546) BpmI (511) BmgBI (471) BsaAI (444) MslI (433) BanII - Bsp1286I (425) BbsI (412) FspI (313) BmeT110I (254) AvaI - BsoBI (253) HaeII - PluTI (146) SfoI (144) NarI (143) KasI (142) TaqII (127) AlwNI (120) BseRI (34) BsaBI * (28) BclI * (11) Start (0) hmUkG1 684 bp
End  (684)
0 sites
BtgI  (641)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
BsiEI  (606)
1 site
C G R Y C G G C Y R G C

Sticky ends from different BsiEI sites may not be compatible.
Bpu10I  (595)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BstXI  (546)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BpmI  (511)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI
recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BmgBI  (471)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BmgBI will not always regenerate a BmgBI site.
BsaAI  (444)
1 site
Y A C G T R R T G C A Y
MslI  (433)
1 site
C A Y N N N N R T G G T R N N N N Y A C
BanII  (425)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
Bsp1286I  (425)
1 site
G D G C H C C H C G D G

Sticky ends from different Bsp1286I sites may not be compatible.
BbsI  (412)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
FspI  (313)
1 site
T G C G C A A C G C G T
BmeT110I  (254)
1 site
C Y C G R G G R G C Y C
AvaI  (253)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (253)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures
up to 65°C.
HaeII  (146)
1 site
R G C G C Y Y C G C G R
PluTI  (146)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
SfoI  (144)
1 site
G G C G C C C C G C G G
NarI  (143)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
KasI  (142)
1 site
G G C G C C C C G C G G
TaqII  (127)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
AlwNI  (120)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BseRI  (34)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
BsaBI  (28)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
BclI  (11)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
Start  (0)
0 sites
hmUkG1
1 .. 684  =  684 bp
227 amino acids  =  25.9 kDa
Product: humanized monomeric variant of
Umikinoko-Green fluorescent protein
mammalian codon-optimized
hmUkG1
1 .. 684  =  684 bp
227 amino acids  =  25.9 kDa
Product: humanized monomeric variant of
Umikinoko-Green fluorescent protein
mammalian codon-optimized
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