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Plasmid Files

mRuby

Monomeric variant of the red fluorescent protein eqFP611.

 
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 600 400 200 End (681) NaeI (666) NgoMIV (664) BsgI (655) MmeI (638) EarI (602) XcmI (546) PstI (517) SfcI (513) Bpu10I (508) AcuI - Eco57MI (505) BanII (425) HaeII - PluTI (392) SfoI (390) BsaHI - NarI (389) KasI (388) ApoI (372) BmeT110I (254) AvaI - BsoBI (253) SgrAI (79) ApaLI (75) NspI (27) Start (0) mRuby 681 bp
End  (681)
0 sites
NaeI  (666)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI
recognition sequence.
NgoMIV  (664)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV
recognition sequence.
BsgI  (655)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI
recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
MmeI  (638)
1 site
T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18

Efficient cleavage requires at least two copies of the MmeI
recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
EarI  (602)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI
recognition sequence.
Sticky ends from different EarI sites may not be compatible.
XcmI  (546)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
PstI  (517)
1 site
C T G C A G G A C G T C
SfcI  (513)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long
incubations.
Bpu10I  (508)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
AcuI  (505)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the AcuI
recognition sequence.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
Eco57MI  (505)
1 site
C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14

Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BanII  (425)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
HaeII  (392)
1 site
R G C G C Y Y C G C G R
PluTI  (392)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
SfoI  (390)
1 site
G G C G C C C C G C G G
BsaHI  (389)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
NarI  (389)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
KasI  (388)
1 site
G G C G C C C C G C G G
ApoI  (372)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
BmeT110I  (254)
1 site
C Y C G R G G R G C Y C
AvaI  (253)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (253)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures
up to 65°C.
SgrAI  (79)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI
recognition sequence.
ApaLI  (75)
1 site
G T G C A C C A C G T G
NspI  (27)
1 site
R C A T G Y Y G T A C R
Start  (0)
0 sites
mRuby
1 .. 681  =  681 bp
227 amino acids  =  25.2 kDa
Product: monomeric variant of the red fluorescent
protein eqFP611 (Kredel et al., 2009)
mRuby
1 .. 681  =  681 bp
227 amino acids  =  25.2 kDa
Product: monomeric variant of the red fluorescent
protein eqFP611 (Kredel et al., 2009)
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