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Plasmid Files

mWasabi

Green variant of the teal fluorescent protein mTFP1.

 
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 600 400 200 mWasabi End (711) BsrGI - TatI (701) XcmI (687) BstEII (645) BstYI (614) DraIII (597) BglI (568) SfcI (555) HincII (540) AatII (497) ZraI (495) BsaAI (468) PspFI (444) BseYI - PflMI (440) BsrFI (436) SmlI (385) BpuEI (370) MmeI (365) NcoI - StyI (350) MslI (349) BmeT110I (278) AvaI - BsoBI (277) EcoO109I (224) BanII - Bsp1286I (171) BstXI (150) BanI (133) MscI (86) Start (0) mWasabi 711 bp
End  (711)
0 sites
BsrGI  (701)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
TatI  (701)
1 site
W G T A C W W C A T G W
XcmI  (687)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
BstEII  (645)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BstYI  (614)
1 site
R G A T C Y Y C T A G R
DraIII  (597)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
BglI  (568)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
SfcI  (555)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long
incubations.
HincII  (540)
1 site
G T Y R A C C A R Y T G
AatII  (497)
1 site
G A C G T C C T G C A G
ZraI  (495)
1 site
G A C G T C C T G C A G
BsaAI  (468)
1 site
Y A C G T R R T G C A Y
PspFI  (444)
1 site
C C C A G C G G G T C G
BseYI  (440)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
PflMI  (440)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
BsrFI  (436)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI
recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its
electrophoretic mobility.
SmlI  (385)
1 site
C T Y R A G G A R Y T C

Efficient cleavage requires at least two copies of the SmlI
recognition sequence.
Sticky ends from different SmlI sites may not be compatible.
BpuEI  (370)
1 site
C T T G A G ( N ) 14 N N G A A C T C ( N ) 14

Sticky ends from different BpuEI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
MmeI  (365)
1 site
T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18

Efficient cleavage requires at least two copies of the MmeI
recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
NcoI  (350)
1 site
C C A T G G G G T A C C
StyI  (350)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
MslI  (349)
1 site
C A Y N N N N R T G G T R N N N N Y A C
BmeT110I  (278)
1 site
C Y C G R G G R G C Y C
AvaI  (277)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (277)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures
up to 65°C.
EcoO109I  (224)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BanII  (171)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
Bsp1286I  (171)
1 site
G D G C H C C H C G D G

Sticky ends from different Bsp1286I sites may not be compatible.
BstXI  (150)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BanI  (133)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
MscI  (86)
1 site
T G G C C A A C C G G T
Start  (0)
0 sites
mWasabi
1 .. 711  =  711 bp
236 amino acids  =  26.9 kDa
Product: green variant of teal fluorescent protein
mTFP1 (Ai et al., 2008)
mWasabi
1 .. 711  =  711 bp
236 amino acids  =  26.9 kDa
Product: green variant of teal fluorescent protein
mTFP1 (Ai et al., 2008)
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