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Plasmid Files

miniSOG

Mini singlet oxygen generator, a fluorescent flavoprotein suitable for correlated light and electron microscopy.

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miniSOG Sequence and MapminiSOG.dna
Map and Sequence File   
Sequence Author:  Tsien Lab
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 300 200 100 miniSOG End (321) MslI (301) ScaI (292) BstAPI (264) AarI (263) BsrBI (227) BsaHI (173) BmeT110I (137) ApaI - AvaI - BaeGI - BanII - Bme1580I - BsoBI - Bsp1286I (136) BsaI (133) PspOMI (132) SbfI (131) BsiEI (120) EaeI - EagI (117) BglI - SfiI (116) BseRI (112) BpmI - Eco57MI * (94) PfoI * (71) XmnI (10) BspQI - EarI - SapI (1) Start (0) miniSOG 321 bp
End  (321)
0 sites
MslI  (301)
1 site
C A Y N N N N R T G G T R N N N N Y A C
ScaI  (292)
1 site
A G T A C T T C A T G A
BstAPI  (264)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
AarI  (263)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI
recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its
electrophoretic mobility.
BsrBI  (227)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures
up to 50°C.
BsaHI  (173)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
BmeT110I  (137)
1 site
C Y C G R G G R G C Y C
ApaI  (136)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
AvaI  (136)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BaeGI  (136)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
BanII  (136)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
Bme1580I  (136)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
BsoBI  (136)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures
up to 65°C.
Bsp1286I  (136)
1 site
G D G C H C C H C G D G

Sticky ends from different Bsp1286I sites may not be compatible.
BsaI  (133)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
PspOMI  (132)
1 site
G G G C C C C C C G G G
SbfI  (131)
1 site
C C T G C A G G G G A C G T C C
BsiEI  (120)
1 site
C G R Y C G G C Y R G C

Sticky ends from different BsiEI sites may not be compatible.
EaeI  (117)
1 site
Y G G C C R R C C G G Y
EagI  (117)
1 site
C G G C C G G C C G G C
BglI  (116)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
SfiI  (116)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI
recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
BseRI  (112)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
BpmI  (94)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI
recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI quickly loses activity at 37°C.
Eco57MI  (94)
1 site
C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14
* Blocked by Dcm methylation.
Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
PfoI  (71)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
XmnI  (10)
1 site
G A A N N N N T T C C T T N N N N A A G
BspQI  (1)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
EarI  (1)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI
recognition sequence.
Sticky ends from different EarI sites may not be compatible.
SapI  (1)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be
mixed before removing an aliquot.
Start  (0)
0 sites
miniSOG
1 .. 321  =  321 bp
106 amino acids  =  12.3 kDa
Product: mini singlet oxygen generator, a
fluorescent flavoprotein suitable for correlated light
and electron microscopy (Shu et al., 2011)
miniSOG
1 .. 321  =  321 bp
106 amino acids  =  12.3 kDa
Product: mini singlet oxygen generator, a
fluorescent flavoprotein suitable for correlated light
and electron microscopy (Shu et al., 2011)
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