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Plasmid Files

pDsRed-Monomer-N In-Fusion® Ready

Linearized vector for fusing DsRed-Monomer to the C-terminus of a partner protein.

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pDsRed-Monomer-N In-Fusion Ready Sequence and MappDsRed-Monomer-N In-Fusion Ready.dna
Map and Sequence File   
Sequence Author:  Clontech
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 AflIII - PciI (3986) ApaLI (3672) EcoO109I (3166) BsaI (3057) PfoI (2843) BstBI (2750) RsrII (2584) BsrDI (2301) PflFI - Tth111I (2186) MscI (2150) PluTI (2071) SfoI (2069) NarI (2068) KasI (2067) BspDI * - ClaI * (1908) SfiI (1843) SexAI * (1657) AseI (4051) NdeI (4278) SnaBI (4384) NheI (4635) BmtI (4639) Bpu10I (4640) End (4655) Start (0) EcoRI (8) AgeI (20) FspAI (75) BstEII (167) AhdI (206) AvaI - BsoBI (288) BmeT110I (289) AfeI (309) SbfI (367) BbsI (447) BglII (505) BsrGI (565) PflMI (608) AleI (654) NotI (706) XbaI * (722) MfeI (818) HincII - HpaI (831) Bts α I (907) AflII (950) pDsRed-Monomer-N In-Fusion® Ready 4655 bp
AflIII  (3986)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (3986)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
ApaLI  (3672)
1 site
G T G C A C C A C G T G
EcoO109I  (3166)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BsaI  (3057)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
PfoI  (2843)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
BstBI  (2750)
1 site
T T C G A A A A G C T T
RsrII  (2584)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII
recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2301)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2186)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2186)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
MscI  (2150)
1 site
T G G C C A A C C G G T
PluTI  (2071)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
SfoI  (2069)
1 site
G G C G C C C C G C G G
NarI  (2068)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
KasI  (2067)
1 site
G G C G C C C C G C G G
BspDI  (1908)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (1908)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
SfiI  (1843)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI
recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
SexAI  (1657)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
AseI  (4051)
1 site
A T T A A T T A A T T A
NdeI  (4278)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
SnaBI  (4384)
1 site
T A C G T A A T G C A T
NheI  (4635)
1 site
G C T A G C C G A T C G
BmtI  (4639)
1 site
G C T A G C C G A T C G
Bpu10I  (4640)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
End  (4655)
0 sites
Start  (0)
0 sites
EcoRI  (8)
1 site
G A A T T C C T T A A G
AgeI  (20)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
FspAI  (75)
1 site
R T G C G C A Y Y A C G C G T R
BstEII  (167)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
AhdI  (206)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AvaI  (288)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (288)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures
up to 65°C.
BmeT110I  (289)
1 site
C Y C G R G G R G C Y C
AfeI  (309)
1 site
A G C G C T T C G C G A
SbfI  (367)
1 site
C C T G C A G G G G A C G T C C
BbsI  (447)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BglII  (505)
1 site
A G A T C T T C T A G A
BsrGI  (565)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
PflMI  (608)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
AleI  (654)
1 site
C A C N N N N G T G G T G N N N N C A C
NotI  (706)
1 site
G C G G C C G C C G C C G G C G
XbaI  (722)
1 site
T C T A G A A G A T C T
* Blocked by Dam methylation.
MfeI  (818)
1 site
C A A T T G G T T A A C
HincII  (831)
1 site
G T Y R A C C A R Y T G
HpaI  (831)
1 site
G T T A A C C A A T T G
BtsαI  (907)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsI sites may not be compatible.
AflII  (950)
1 site
C T T A A G G A A T T C

The sticky ends produced by AflII are hard to ligate.
NeoR/KanR
1940 .. 2734  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin)
NeoR/KanR
1940 .. 2734  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin)
DsRed-Monomer
30 .. 704  =  675 bp
224 amino acids  =  25.3 kDa
Product: monomeric derivative of DsRed fluorescent
protein (Strongin et al., 2007)
mammalian codon-optimized
DsRed-Monomer
30 .. 704  =  675 bp
224 amino acids  =  25.3 kDa
Product: monomeric derivative of DsRed fluorescent
protein (Strongin et al., 2007)
mammalian codon-optimized
ori
3342 .. 3930  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
3342 .. 3930  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
f1 ori
960 .. 1415  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
960 .. 1415  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
SV40 promoter
1548 .. 1905  =  358 bp
SV40 enhancer and early promoter
SV40 promoter
1548 .. 1905  =  358 bp
SV40 enhancer and early promoter
CMV enhancer
4105 .. 4408  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
4105 .. 4408  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
4409 .. 4612  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
4409 .. 4612  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
SV40 poly(A) signal
832 .. 953  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
832 .. 953  =  122 bp
SV40 polyadenylation signal
AmpR promoter
1442 .. 1546  =  105 bp
AmpR promoter
1442 .. 1546  =  105 bp
HSV TK poly(A) signal
2966 .. 3013  =  48 bp
herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)
HSV TK poly(A) signal
2966 .. 3013  =  48 bp
herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)
SV40 ori
1756 .. 1891  =  136 bp
SV40 origin of replication
SV40 ori
1756 .. 1891  =  136 bp
SV40 origin of replication
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