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Plasmid Files

pEGFP-C2

Vector for fusing EGFP to the N-terminus of a partner protein. For other reading frames, use pEGFP‑C1 or pEGFP‑C3.

To see this sequence with restriction sites, features, and translations, please download
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pEGFP-C2.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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PciI (4677) ApaLI (4363) EcoO109I (3857) BsaI (3748) RsrII (3275) BsrDI (2992) PflFI - Tth111I (2877) FspI (2861) MscI (2841) PluTI (2762) SfoI (2760) NarI (2759) KasI (2758) BspDI * - ClaI * (2599) StuI (2580) SfiI (2534) AseI (7) NdeI (234) SnaBI (340) NheI (591) BmtI (595) AfeI (596) AgeI (600) BsrGI (1322) BglII (1343) PaeR7I - XhoI (1347) Eco53kI (1352) SacI (1354) HindIII (1356) EcoRI (1363) PstI (1372) SalI (1373) AccI (1374) Acc65I (1379) KpnI (1383) SacII (1386) PspOMI (1387) TspMI - XmaI (1390) ApaI (1391) SmaI (1392) BamHI (1394) XbaI * (1406) BclI * (1416) MfeI (1509) HpaI (1522) MluI (1645) DraIII (1875) SexAI * (2348) pEGFP-C2 4735 bp
PciI  (4677)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
ApaLI  (4363)
1 site
G T G C A C C A C G T G
EcoO109I  (3857)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BsaI  (3748)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
RsrII  (3275)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2992)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2877)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2877)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FspI  (2861)
1 site
T G C G C A A C G C G T
MscI  (2841)
1 site
T G G C C A A C C G G T
PluTI  (2762)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (2760)
1 site
G G C G C C C C G C G G
NarI  (2759)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (2758)
1 site
G G C G C C C C G C G G
BspDI  (2599)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2599)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
StuI  (2580)
1 site
A G G C C T T C C G G A
SfiI  (2534)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AseI  (7)
1 site
A T T A A T T A A T T A
NdeI  (234)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (340)
1 site
T A C G T A A T G C A T
NheI  (591)
1 site
G C T A G C C G A T C G
BmtI  (595)
1 site
G C T A G C C G A T C G
AfeI  (596)
1 site
A G C G C T T C G C G A
AgeI  (600)
1 site
A C C G G T T G G C C A
BsrGI  (1322)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
BglII  (1343)
1 site
A G A T C T T C T A G A
PaeR7I  (1347)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1347)
1 site
C T C G A G G A G C T C
Eco53kI  (1352)
1 site
G A G C T C C T C G A G
SacI  (1354)
1 site
G A G C T C C T C G A G
HindIII  (1356)
1 site
A A G C T T T T C G A A
EcoRI  (1363)
1 site
G A A T T C C T T A A G
PstI  (1372)
1 site
C T G C A G G A C G T C
SalI  (1373)
1 site
G T C G A C C A G C T G
AccI  (1374)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.