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Plasmid Files

pTagRFP-C

Vector for fusing TagRFP to the N-terminus of a partner protein.

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pTagRFP-C Sequence and MappTagRFP-C.dna
Map and Sequence File
Sequence Author:  Evrogen
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 PciI (4667) EcoO109I (3847) RsrII (3265) BsrDI (2982) PflFI - Tth111I (2867) FspI (2851) PluTI (2752) SfoI (2750) NarI (2749) KasI (2748) EagI (2655) BspDI * - ClaI * (2589) StuI (2570) BseRI (2567) SfiI (2524) SV40 promoter AseI (7) NdeI (234) SnaBI (340) NheI (591) BmtI (595) AfeI (596) AgeI (600) AleI (613) BsrGI (662) BbsI (920) PflMI (1218) PshAI (1252) BspEI (1324) BglII (1333) PaeR7I - XhoI (1337) Eco53kI (1342) SacI (1344) HindIII (1346) EcoRI (1353) PstI (1362) SalI (1363) Acc65I (1369) KpnI (1373) SacII (1376) PspOMI (1377) TspMI - XmaI (1380) ApaI (1381) SmaI (1382) BamHI (1384) XbaI * (1396) BclI * (1406) MfeI (1499) HpaI (1512) Bts α I (1588) MluI (1635) SexAI * (2338) pTagRFP-C 4725 bp
PciI  (4667)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
EcoO109I  (3847)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
RsrII  (3265)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII
recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2982)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2867)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2867)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FspI  (2851)
1 site
T G C G C A A C G C G T
PluTI  (2752)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
SfoI  (2750)
1 site
G G C G C C C C G C G G
NarI  (2749)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
KasI  (2748)
1 site
G G C G C C C C G C G G
EagI  (2655)
1 site
C G G C C G G C C G G C
BspDI  (2589)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2589)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
StuI  (2570)
1 site
A G G C C T T C C G G A
BseRI  (2567)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
SfiI  (2524)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI
recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AseI  (7)
1 site
A T T A A T T A A T T A
NdeI  (234)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
SnaBI  (340)
1 site
T A C G T A A T G C A T
NheI  (591)
1 site
G C T A G C C G A T C G
BmtI  (595)
1 site
G C T A G C C G A T C G
AfeI  (596)
1 site
A G C G C T T C G C G A
AgeI  (600)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
AleI  (613)
1 site
C A C N N N N G T G G T G N N N N C A C
BsrGI  (662)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
BbsI  (920)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
PflMI  (1218)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
PshAI  (1252)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
BspEI  (1324)
1 site
T C C G G A A G G C C T
BglII  (1333)
1 site
A G A T C T T C T A G A
PaeR7I  (1337)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1337)
1 site
C T C G A G G A G C T C
Eco53kI  (1342)
1 site
G A G C T C C T C G A G
SacI  (1344)
1 site
G A G C T C C T C G A G
HindIII  (1346)
1 site
A A G C T T T T C G A A
EcoRI  (1353)
1 site
G A A T T C C T T A A G
PstI  (1362)
1 site
C T G C A G G A C G T C
SalI  (1363)
1 site
G T C G A C C A G C T G
Acc65I  (1369)
1 site
G G T A C C C C A T G G
KpnI  (1373)
1 site
G G T A C C C C A T G G
SacII  (1376)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
PspOMI  (1377)
1 site
G G G C C C C C C G G G
TspMI  (1380)
1 site
C C C G G G G G G C C C
XmaI  (1380)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
ApaI  (1381)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
SmaI  (1382)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
BamHI  (1384)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
XbaI  (1396)
1 site
T C T A G A A G A T C T
* Blocked by Dam methylation.
BclI  (1406)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
MfeI  (1499)
1 site
C A A T T G G T T A A C
HpaI  (1512)
1 site
G T T A A C C A A T T G
BtsαI  (1588)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsI sites may not be compatible.
MluI  (1635)
1 site
A C G C G T T G C G C A
SexAI  (2338)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
NeoR/KanR
2621 .. 3415  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin)
NeoR/KanR
2621 .. 3415  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin)
TagRFP
613 .. 1323  =  711 bp
237 amino acids  =  26.7 kDa
Product: monomeric derivative of red fluorescent
protein from Entacmaea quadricolor
mammalian codon-optimized
TagRFP
613 .. 1323  =  711 bp
237 amino acids  =  26.7 kDa
Product: monomeric derivative of red fluorescent
protein from Entacmaea quadricolor
mammalian codon-optimized
ori
4023 .. 4611  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
4023 .. 4611  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
f1 ori
1641 .. 2096  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
1641 .. 2096  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
SV40 promoter
2229 .. 2586  =  358 bp
SV40 enhancer and early promoter
SV40 promoter
2229 .. 2586  =  358 bp
SV40 enhancer and early promoter
CMV enhancer
61 .. 364  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
61 .. 364  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
365 .. 568  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
365 .. 568  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
SV40 poly(A) signal
1513 .. 1634  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1513 .. 1634  =  122 bp
SV40 polyadenylation signal
AmpR promoter
2123 .. 2227  =  105 bp
AmpR promoter
2123 .. 2227  =  105 bp
MCS
1324 .. 1389  =  66 bp
multiple cloning site of fluorescent protein plasmids
MCS
1324 .. 1389  =  66 bp
multiple cloning site of fluorescent protein plasmids
HSV TK poly(A) signal
3647 .. 3694  =  48 bp
herpesvirus thymidine kinase polyadenylation signal
HSV TK poly(A) signal
3647 .. 3694  =  48 bp
herpesvirus thymidine kinase polyadenylation signal
SV40 ori
2437 .. 2572  =  136 bp
SV40 origin of replication
SV40 ori
2437 .. 2572  =  136 bp
SV40 origin of replication
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