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Plasmid Files

pTurboRFP-B

Vector for expressing TurboRFP in bacteria.

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pTurboRFP-B.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Evrogen
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AvaI - BsoBI - PaeR7I - XhoI (1) EcoO109I (4086) AatII (4032) ZraI (4030) XmnI (3709) PvuI (3480) FspI (3332) NmeAIII (3258) BsrFI (3190) AhdI (3110) AlwNI (2633) AflIII - PciI (2217) BspQI - SapI (2101) BstAPI (2041) BmeT110I (2) PsiI (49) MfeI (59) BseRI (115) BsaBI * (125) BamHI (126) BclI * (142) BsrGI (169) Bsu36I (385) SfiI (595) BsgI (624) BfuAI - BspMI (646) DraIII (713) PshAI (759) HindIII (827) BlpI (839) NheI (947) BmtI (951) Bpu10I (972) PvuII (1099) BspEI (1195) BtgI - NcoI - StyI (1500) XbaI (1804) PfoI (1861) PflFI - Tth111I (1964) BsaAI (1971) AccI (1989) BstZ17I (1990) NdeI (2040) pTurboRFP-B 4102 bp
AvaI  (1)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (1)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
PaeR7I  (1)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1)
1 site
C T C G A G G A G C T C
EcoO109I  (4086)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
AatII  (4032)
1 site
G A C G T C C T G C A G
ZraI  (4030)
1 site
G A C G T C C T G C A G
XmnI  (3709)
1 site
G A A N N N N T T C C T T N N N N A A G
PvuI  (3480)
1 site
C G A T C G G C T A G C
FspI  (3332)
1 site
T G C G C A A C G C G T
NmeAIII  (3258)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BsrFI  (3190)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
AhdI  (3110)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (2633)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
AflIII  (2217)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (2217)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (2101)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (2101)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BstAPI  (2041)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
BmeT110I  (2)
1 site
C Y C G R G G R G C Y C
PsiI  (49)
1 site
T T A T A A A A T A T T
MfeI  (59)
1 site
C A A T T G G T T A A C
BseRI  (115)
1 site
G A G G A G ( N