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Plasmid Files

smURFP

Dimeric small ultra-red fluorescent protein derived from a cyanobacterial phycobiliprotein.

 
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smURFP.dna
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 400 300 200 100 End (402) BsaI (352) BsaHI (350) EarI (338) BstAPI (308) BmrI (280) ApaI - BanII (223) PspOMI (219) EaeI (206) TaqII (116) EciI (108) AlwNI * (87) BstYI (84) Bts α I (37) Start (0) smURFP 402 bp
End  (402)
0 sites
BsaI  (352)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BsaHI  (350)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
EarI  (338)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI
recognition sequence.
Sticky ends from different EarI sites may not be compatible.
BstAPI  (308)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
BmrI  (280)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the
absence of magnesium.
ApaI  (223)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
BanII  (223)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
PspOMI  (219)
1 site
G G G C C C C C C G G G
EaeI  (206)
1 site
Y G G C C R R C C G G Y
TaqII  (116)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
EciI  (108)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
AlwNI  (87)
1 site
C A G N N N C T G G T C N N N G A C
* Blocked by Dcm methylation.
Sticky ends from different AlwNI sites may not be compatible.
BstYI  (84)
1 site
R G A T C Y Y C T A G R
BtsαI  (37)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsI sites may not be compatible.
Start  (0)
0 sites
smURFP
1 .. 402  =  402 bp
133 amino acids  =  15.0 kDa
Product: dimeric small ultra-red fluorescent protein
derived from a cyanobacterial phycobiliprotein
(Rodriguez et al., 2016)
smURFP
1 .. 402  =  402 bp
133 amino acids  =  15.0 kDa
Product: dimeric small ultra-red fluorescent protein
derived from a cyanobacterial phycobiliprotein
(Rodriguez et al., 2016)
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